Sequences from confirmation amplicon


Confirmation sequences for line 164G02 and hit At1g03620 are shown.
Line ID 164G02
Locus At1g03620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:904480
Primers used for PCR 38pm - 8474, show primer details
   
Sequence Name 40-K115064-0022-164-G02C-38pm
GenBank Accession LN503808 [GenBank]
Sequencing primer gene specific primer, 38pm: 5'-TACCAGTTCATAATTTCCACACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K115064-0022-164-G02C-38pm
ATAGAACATACGTTCATTATCATTCATTTTCTATTTATGTGTTTTGCTTCATTGATTATC
ATTTTCATTTCGGTCATAAATTCAGGTTAATGTACAAAACATAAAAATCGAAATCAAACT
AAGAAAATAACAAAAGAAAAAAAAATCTATTGGAACAAGAGATTGTAGGCAGGAAGATCT
TGAATCCGATGAATATCATCCAAAGAAAGCTCTCTCTCCAGCTGATTCCGAGTTGCTTGT
AATACTTCCTGATAAACACACACAGATATATACAATTATTAAGAACTAATTCTCAAAAAA
AAAAAAAATGGAAAAAAAAATTTTAATAATTGGGGGGAAAAAAAATCCTTTAATTTCCTG
GTAAAAAGGGGGCTTTGAAGCCCTTTGACCTCCCTTCACCGCAAAGCCTATCCGTTAAAT
AACTCCAAAGGCCCCCCCGTCTTCGGTAAACCATGGAAAAAAAATTCTGGATTATTAGGG
GATGGTCAAACAACACTACAAATTTTAAAAAAAAGGGTTTTTTTCTTTTTAAAAAACCTT
TTTAAAAAAACCAAAAAATTTTGGGGGTTTCTTTTCGGGAAATTCTCCGGGGATCACCAT
GGATTAGAAGGGTAAATTGGGAAAAAAAAAAAAATTATTTCCCATTTTTTTTTCATTTCA
AAATTGTAAATTC
   
Sequence Name 08-K115063-0022-164-G02C-8409
GenBank Accession LN489850 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K115063-0022-164-G02C-8409
CTCTTTTGTTTTTTATATATGTTCTTTTAACTGAATAAAACCTTCTTTCTAAAAGTTAGT
AGTGTAGTATGACGATCGCTAAATAATCAAGAATCATATTCCAATGATTAACAGAAGACG
AGAGGGCATTCGATGTACTATACTGTATAGCTTTCGCGATGATGGATGCTCAATGGCTTG
CAATGCACGCTTCTTACATGGAATTCAATGTATCTCTTCTCACTCAATCTATCAACTTTC
TCTTTTCATTTTTTTTTTTTTTGGAAAATTATTTCTAAAAAATGGTAAATTTCTGGGGGG
GTTTACCGGGAATTTTTACAGCCACCTCGAATTCCCCGGGAAAAAGACTTTTTTTGGGAG
AATTTTCACGGAATTAAAAATTTTCCGGCCAACAATCTTTGGTCCAAAAAAATTTTTTTT
CCTTTGGTAATTTCCTAAGTTGGATTCCAATTTTAAGTTTTGGACCTTAACCCGGATTTT
TTCCCCAAATTGAAATTGATTTTCATGGAGCCAAACCCCTTAATTGAAAATGGAAGGATA
AGGAAGTTTTTTTCTATTTAAGCCACCATTTTTGGGAAACAGAAAAAAAAGGGTGGGAAA

Last Updated on 06.12.2018 16:11