Sequences from confirmation amplicon


Confirmation sequences for line 165A11 and hit At5g19190 are shown.
Line ID 165A11
Locus At5g19190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6457773
Primers used for PCR 02mm - 8474, show primer details
   
Sequence Name 43-K114393-0022-165-A11C-02mm
GenBank Accession LN504923 [GenBank]
Sequencing primer gene specific primer, 02mm: 5'-GGAGAGAGAGAGAGAGACATGGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K114393-0022-165-A11C-02mm
TCCTCTATAATCTTGTTGTCATCAACCAACAAATTTGATTTTCATTCAATCCTTAAAAGA
TTACAAATGGAGGAAAAAGTTAGTGCATCTCTAAAATGACAAGAAAGAGTTACATAAAAG
CAAGAACAAACTTGGAAGTTGGAACACATCTCCAATACCTTCCTTATACAACCCTTTTAT
TGTTCGCTGAATTTACAAACAAATAAATCTTATGCATCTAATTTAAATTATATGAATTGA
TCACACAGAAGAATATTTGTTAAAATTAATCAAGTGTAGATAGATGATGCATCTCTGTAG
CTTCTTCTTGGGATAGAAGCATGAACACTAGTGCTGTTCATGTTGCTCTTGTTGTTGTTA
TTGTTGTGAGTGGTGAGAGTACTTCTTGAGTTCTCATGATTTTGCTGATACTCTTCAATT
GTAGGTTTGGCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATATCCGCACGGTTA
   
Sequence Name 19-K114392-0022-165-A11C-8409
GenBank Accession LN494860 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K114392-0022-165-A11C-8409
TATATCCTGGGCAAACCTACAATTGAAGAGTATCAGCAAAATCATGAGAACTCAAGAAGT
ACTCTCACCACTCACAACAATAACAACAACAAGAGCAACATGAACAGCACTAGTGTTCAT
GCTTCTATCCCAAGAAGAAGCTACAGAGATGCATCATCTATCTACACTTGATTAATTTTA
ACAAATATTCTTCTGTGTGATCAATTCATATAATTTAAATTAGATGCATAAGATTTATTT
GTTTGTAAATTCAGCGAACAATAAAAGGGTTGTATAAGGAAGGTATTGGAGATGTGTTCC
AACTTCCAAGTTTGTTCTTGCTTTTATGTAACTCTTTCTTGTCATTTTAGAGATGCACTA
ACTTTTTCCTCCATTTGTAATCTTTTAAGGATTGAATGAAAATCAAATTTGTTGGTTGAT
GACAACAAGATTGATAGAGGAATGTGTAAAGACAAGCCCCATGTGAGATTAGCAAATGTT
CCATGTCTCTCTCTCTCTC

Last Updated on 06.12.2018 16:11