Sequences from confirmation amplicon


Confirmation sequences for line 165C11 and hit At5g65700 are shown.
Line ID 165C11
Locus At5g65700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26282930
Primers used for PCR NK35 - 8474, show primer details
   
Sequence Name 15-K102660-0022-165-C11C-8409
GenBank Accession LN492924 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K102660-0022-165-C11C-8409
CATTTACATTGAATATATATACTCATTGCTGATCCAATAAGCTGGAAGAGAGATCGACGA
GATTTAGTTTACCGTTTTCTCCTAATTTCTGCGGGATGCTTCCGGTGAAATTGTTCTCCC
AAAGCTGTAACACTTCAAGCTCCGGCAAATCTCCGATGAACTCCGGTATCTCGCCGTGAA
GTTTGTTACGGAAGAGATTCAAAAGCGTGAGATTCTTCAACTCTGCGAAACTCGCTGGAA
TCTCTCCGGTGAACATGTTGTTAGACAAATCCATTGATTTTAAACTCGATAGCGTTCCTA
GCTCCCAAGTTAATGGACCGGAGAAGACATTCACTTGCAAGAAAAGCGTATCGAGTTTCT
GAAGCTTCCCAATCTCCGGCGGAATCTCACCGGTTAATCCGCAATTAGCTCCGTCGAATC
TGACTAGCTCCGATAGATTTCCGATCTCTGGAGGAAGACCGTCTTCGAAAGCGTTGTAGT
AGCCGATGTAAAGCTCCCGGAGAGTCGTTAGGTTTCCGATCTCTGGAGGGATTTTCCCGA
CAAGCTCGTTGCCGGAAACCGCTAGATACTCAATAACTGGCCAGCTT
   
Sequence Name 34-K102661-0022-165-C11C-NK35
GenBank Accession LN501371 [GenBank]
Sequencing primer gene specific primer, NK35: 5'-GAAGCTGGCCAGTTATTGAGTATC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K102661-0022-165-C11C-NK35
GGAATCCCTCCGAGATCGGAAACCTAACGACTCTCCGGGAGCTTTACATCGGCTACTACA
ACGCTTTCGAAGACGGTCTTCCTCCAGAGATCGGAAATCTATCGGAGCTAGTCAGATTCG
ACGGAGCTAATTGCGGATTAACCGGTGAGATTCCGCCGGAGATTGGGAAGCTTCAGAAAC
TCGATACGCTTTTCTTGCAAGTGAATGTCTTCTCCGGTCCATTAACTTGGGAGCTAGGAA
CGCTATCGAGTTTAAAATCAATGGATTTGTCTAACAACATGTTCACCGGAGAGATTCCAG
CGAGTTTCGCAGAGTTGAAGAATCTCACGCTTTTGAATCTCTTCCGTAACAAACTTCACG
GCGAGATACCGGAGTTCATCGGAGATTTGCCGGAGCTTGAAGTGTTACAGCTTTGGGAGA
ACAATTTCACCGGAAGCATCCCGCAGAAATTAGGAGAAAACGGTAAACTAAATCTCGTCG
ATCTCTCTTCCAGCTTATTGGATCAGCAATGAGTATATATATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAA
ATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCA
ATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTACT
Primers used for PCR 56wt - 8474, show primer details
   
Sequence Name 72-K118656-0022-165-C11C-56wt
GenBank Accession LN512807 [GenBank]
Sequencing primer gene specific primer, 56wt: 5'-AGATAAGGACCACAGAGATCAGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K118656-0022-165-C11C-56wt
GTACTGAATGTCCTGTTCCCGGAACTAACCGGAGAGGTTGTTGTAAGAGAAATCAAGAGA
TGTTAAGCTCTGCATTGACGAGATTGAACCAGGGATTGATCCAACCAGATGGTTTCTCGA
CAGGTTCAAGTAATTCAATATCTTCATAGCAGTGATCTCATTTGGGATTTCACCGGAGAG
CTCGTTTCTGCTCAGATCAACAAACGTTAAGAGCTTGCAACGACTAATCTCCGGCGCGAT
TCGACCGGAGAACAAGTTGTGGCTGAAATCAATCTTCGAGAGCTGCTGAAGCTTCCCTAC
CTCTGAAGGAATAGGACCTTGGAACTTGTTTCCATCAAGAAGAAGTTTCTGAACGCCGGT
GAAGTTCCCGATCGCCGGAGGTAATGGACCTGAGAGCTGGTTGTTTGATAAACTGATCTG
ACCAAGATTAACAGAGACACCTCCGGCGACTGGTAACTCTCCGGAGAGATAATTATCTTG
GAGCTCCACTTGAGTTAATTTGGGTAATCCGAATAGTCCTTTTGGGATTGACCCGTTCAG
GAAATTCTCACCCATTCGGATCCGGGTCAAAGACTCACATTTACCAAGAGAATCAGGGAT
TGAACCAAAGAGAAAGTTTCCAAGAGTGATTAAAGTTTCTAACTTGTTACCGGAGCACAT
GTTCGGCGGTAAAGAGAAAAAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11