Genotyping and primer details


Genotyping and primer details for 165C11 - At5g65700
Line ID 165C11
Locus At5g65700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26282930 of line 165C11 Chr5:26282930
   
Insertion allele amplicon 8474 - 56wt
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 56wt: 5'-AGATAAGGACCACAGAGATCAGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26283672
   
Insertion allele amplicon 8474 - NK35
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NK35: 5'-GAAGCTGGCCAGTTATTGAGTATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26282385
   
wt amplicon (over InsPos) MN57 - NK35
wt amplicon start Chr5:26282385
wt amplicon end Chr5:26283314
wt amplicon size 930 bp
Locus specific primer NK35: 5'-GAAGCTGGCCAGTTATTGAGTATC-3'
2nd locus specific primer MN57: 5'-AAGGAATAGGACCTTGGAACTTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11