Sequences from confirmation amplicon


Confirmation sequences for line 165E10 and hit At5g08600 are shown.
Line ID 165E10
Locus At5g08600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2788515
Primers used for PCR 03md - 8474, show primer details
   
Sequence Name 47-K114180-0022-165-E10C-03md
GenBank Accession LN506266 [GenBank]
Sequencing primer gene specific primer, 03md: 5'-CAAATTCATGCTACTTTGTCCAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K114180-0022-165-E10C-03md
GATGAGGAAGATTTGAATGATGGTTCAGATCAGGATACTTCTAAACTGATAGCGGAAGCA
AAGGAGAAGATACTGAAAACTGTGGACGATGATGAAGTCCCCATTTCTCGTCTTATGTCA
TTACCTTTCATGGTAATTCTTGCTTTGTTTTCTCAAAACACTTTTGAATGTTTTTATTCT
GGTTTTCTTTTCCCGCTCATTCATGTTTTTGGTTTGGTTAGGCTCGTGCTGTGAAAAAGA
AAAACGAGGAAGCTAAAGAAGAAGGTAAACGTGATATTGAGGAGTTGGAGAAATTTGGTG
GAGCAGAAACCTCTAAAAAGTCCACTAATGTTGTTGGTAGAAGAGTATTTGGTGCAGAAG
CTCCAAAAGAGTCTGAAAAGGAATCAGACAACCTTTTTGATAACAGTGATAGTAGTGACA
ATGATATGGAAGATAATGAGTTAGAGGCTGTAAAAGATAATGCTTCACCTGCTAGAAACA
CTGAAACCATTATGGAAGCCGAGGTACGTTCTCCGGTTAGTCATTATGGTTCTTTCTTCT
CTCCCTTTGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCA
   
Sequence Name 11-K114177-0022-165-E10C-8409
GenBank Accession LN491206 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K114177-0022-165-E10C-8409
TTCAAGGGAGAGAAGAAAGAACCATAATGACTAACCGGAGAACGTACCTCGGCTTCCATA
ATGGTTTCAGTGTTTCTAGCAGGTGAAGCATTATCTTTTACAGCCTCTAACTCATTATCT
TCCATATCATTGTCACTACTATCACTGTTATCAAAAAGGTTGTCTGATTCCTTTTCAGAC
TCTTTTGGAGCTTCTGCACCAAATACTCTTCTACCAACAACATTAGTGGACTTTTTAGAG
GTTTCTGCTCCACCAAATTTCTCCAACTCCTCAATATCACGTTTACCTTCTTCTTTAGCT
TCCTCGTTTTTCTTTTTCACAGCACGAGCCTAACCAAACCAAAAACATGAATGAGCGGGA
AAAGAAAACCAGAATAAAAACATTCAAAAGTGTTTTGAGAAAACAAAGCAAGAATTACCA
TGAAAGGTAATGACATAAGACGAGAAATGGGGACTTCATCATCGTCCACAGTTTTCAGTA
TCTTCTCCTTTGCTTCCGCTATCAGTTTAGAAGTATCCTGATCTGAACCATCATTCAAAT
CTTCCTCATCACTACTACTTTCATCTTTTGTGGAGTTCATCTTTCTGGACAAAGTACANN
GAATTTG

Last Updated on 06.12.2018 16:11