Sequences from confirmation amplicon


Confirmation sequences for line 165G06 and hit At3g16040 are shown.
Line ID 165G06
Locus At3g16040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5443684
Primers used for PCR 05qw - 8474, show primer details
   
Sequence Name 82-K115895-0022-165-G06C-05qw
GenBank Accession LN514230 [GenBank]
Sequencing primer gene specific primer, 05qw: 5'-GAAAAGTTGCAGAATTTTTGAAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K115895-0022-165-G06C-05qw
TCTTCTCACATCGCACATGATTTTAATGGTTATAACTTATAAACTAGTCATGGTACATTG
ACAAACATATATAAGGTGATATAGGATTACATTGAGGCAGATTTAGTCTTATTTCTTCCC
ACTTTTCTTAAGTCCTGAGCCTCCGAATGATCCTTTTTGTGAAGCCTTGGCCCTGAGTTC
TTTAAGTGCCTGTTTTCATCACATAAACAAGATCATCAACAGCAATCACCTTCTCTTCGG
GGACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCAAGATTAT
   
Sequence Name 52-K115894-0022-165-G06C-8409
GenBank Accession LN507920 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K115894-0022-165-G06C-8409
ATTGAATATATCCTGTCCCCGAAGAGAAGGTGATTGCTGTTGATGATCTTGTTTATGTGA
TGAAAACAGGCACTTAAAGAACTCAGGGCCAAGGCTTCACAAAAAGGATCATTCGGAGGC
TCAGGACTTAAGAAAAGTGGGAAGAAATAAGACTAAATCTGCCTCAATGTAATCCTATAT
CACCTTATATATGTTTGTCAATGTACCATGACTAGTTTATAAGTTATAACCATTAAAATC
ATGTTGCGATGTTGTAGAAGTAAATATAAATCTAAGACTTGTACCTCCTTCAAAAATTCT
GCAACTTTT

Last Updated on 06.12.2018 16:11