Sequences from confirmation amplicon


Confirmation sequences for line 168A03 and hit At5g58850 are shown.
Line ID 168A03
Locus At5g58850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23765505
Primers used for PCR M119 - 8474, show primer details
   
Sequence Name 28-K014585-022-168-A03C-8409
GenBank Accession LN498620 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K014585-022-168-A03C-8409
CTGTAGATTTCCCGGACATGAAGCCATTTACAATTATTAAACAAAGGAACATAAAGAAAG
AATGATCAAATAACATCACATGATCATGGATGTAGCTAGACCAACGGGAAGCAGATGTTG
CTACCTTGAATAGAACCAGAGAGCATCTCTATCAGATCCATTTCTCTTCTTTCACTTGTA
GAGTTAGCTTGAGGCTCAAGAAACTCGTTGTGGCCTCCATGCTCTACGGTGGTGGATGAC
GTGGAAGATGAGGAAGACGGAAAATGAGTGTCGGAGTATGAAGACGTCGTACCGTTCAAT
AAATAAGATAAGTAGATATCAGACGCAAGGTGGGGTGTATTCGCCGGAGCTGTGATCGTT
GCCTCTTGATGATGAATTCCAACACTGTCGTGCAAGTTAGGGTTAGGGTTCTTGATCATG
AACCCAGATGATGAAGACTGAGTTACCTCATCCGAAGTTTGGCTCAAACCTAAATTCTCA
AGAGAAATCGGATGGTTTGCAAAGATATTTTGAAAGTAATCTAGCTCTTCGTCATATGGA
CCGCCAAGGATGGACGACGCGGAATCACCATCAGAATAGATCTGATCAAGATTAGGTGTA
GAAATAACACTGAGGGTGTTCTCATTCTTCTTCTCGTCAT
   
Sequence Name 29-K014585-022-168-A03C-M119
GenBank Accession LN499068 [GenBank]
Sequencing primer gene specific primer, M119: 5'-ACATTAACAAGGACAATGACGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K014585-022-168-A03C-M119
AACACCATCTGTGTTATTTCTACACCTAATCTTGATCAGATCTATTCTGATGGTGATTCC
GCGTCGTCCATCCTTGGCGGTCCATATGACGAAGAGCTAGATTACTTTCAAAATATCTTT
GCAAACCATCCGATTTCTCTTGAGAATTTAGGTTTGAGCCAAACTTCGGATGAGGTAACT
CAGTCTTCATCATCTGGGTTCATGATCAAGAACCCTAACCCTAACTTGCACGACAGTGTT
GGAATTCATCATCAAGAGGCAACGATCACAGCTCCGGCGAATACACCCCACCTTGCGTCT
GATATCTACTTATCTTATTTATTGAACGGTACGACGTCTTCATACTCCGACACTCATTTT
CCGTCTTCCTCATCTTCCACGTCATCCACCACCGTAGAGCATGGAGGCCACAACGAGTTT
CTTGAGCCTCAAGCTAACTCTACAAGTGAAAGAAGAGAAATGGATCTGATAGAGATGCTC
TCTGGTTCTATTCAAGGTAGCAACATCTGCTTCCCGTTGGTCTAGCTACATCCATGATCA
TGTGATGTTATTTGATCATTCTTTCTTTATGTTCCTTTGTTTAATAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGAT

Last Updated on 06.12.2018 16:11