Sequences from confirmation amplicon


Confirmation sequences for line 168E01 and hit At3g16340 are shown.
Line ID 168E01
Locus At3g16340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5543116
Primers used for PCR 26ci - 8474, show primer details
   
Sequence Name 31-K107934-0022-168-E01C-26ci
GenBank Accession LN500194 [GenBank]
Sequencing primer gene specific primer, 26ci: 5'-TAGGAATGTAAGAGCGAGGGTAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K107934-0022-168-E01C-26ci
TGCCTCCTCCCCGATCCATACCAGTTTGGATCCGTGAAAATATCGAAAATCTCAAGAACA
GCCAACCCTAAGCTCGTGGAGTTATCAGAAGACTGCAAGGAAAGTAAAAGAGCATTGCTT
GATAAAAAAAAAAAAAAAGGGAATTCTTTTAAAAAACTTTTTAATACCTGTACCAAAATT
TTTCCCGGTTGATTAACCCTTCTGGGACCAAGCTTTTCTTTGACATTTAAACCTTCTAAA
GTGTAACCCTTTGGGAAACCCCATTAACCCCTTTTCCCCCTTTTGGGTTTCCCCCCTGTT
ACCCATAACTTCTTCAAAACACAAAATTATTTTTCATTTTAAAAAAATTATTTTGATAGT
GTTTTTGATGTCCTTTTCCCCCTGGGAACAATAACCCCCCCAACCATGTAAATGGCTTCT
TGCCCGGAAAATCTCCTGGGATCACCAATGATTATGAGGGCCAATTTGGAAAAAAAAAAA
AAGTATTTCCCATTTTTTTTTCATTTCAAAAATGTAAATTCCCCCCCCGTTTTTAAAAAA
AANCCCCCGGGGTTGGGGGTGGGGGGTCTTTGGGGTTTTCCGTTTTTT
   
Sequence Name 15-K107933-0022-168-E01C-8409
GenBank Accession LN493004 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K107933-0022-168-E01C-8409
ATTTACTGCTTGGAGGGTTCATTGTTCCTAGAGGTAATAGTACATCAATAACACTATCAA
ACTAATTTTTATAATATGAAAACTAATTTTGTGTTTTGATGATGTTATGGTTAACAGGTG
AGATACCTAAATGGTGGAAATGGGCTTACTGGGTTTCACCAATGGCTTACACTTACGATG
CTTTAACTGTCAATGAAATGCTTGCTCCTAGATGGATTAATCAACCGGTAAAATTTCTGC
TACAGTTATTAACATGTCTCTTATATGACTTCCTTTTTTTTTTTTTTTTATCAAGAAAGG
CTTTTTTATTTTCCTTGCATTTTTCTAAAAACCCCCCAACCTTGGGGTTGGCTGTTCTTA
AAATTTTCAATTTTTTCCCGGACCCAAACGGGTTTTGAATCGGGGAAGGAGGCTTTCCCG
GGTTTACGGTTCTTTTTAAAACCCTCTTTCCCCCCCCTTTAAATTTCAAAA

Last Updated on 06.12.2018 16:11