Sequences from confirmation amplicon


Confirmation sequences for line 168E04 and hit At2g05920 are shown.
Line ID 168E04
Locus At2g05920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:2270063
Primers used for PCR CR04 - 8474, show primer details
   
Sequence Name 41-K017280-022-168-E04C-CR04
GenBank Accession LN503893 [GenBank]
Sequencing primer gene specific primer, CR04: 5'-ATGCTTTCACCTACACACTCAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K017280-022-168-E04C-CR04
ACCAAACCACACAAGAACACTTCCCACTACTTATGGCTCATGATGGGCTTTGGTTTAGCC
TAAACCCAATCAAGATAACTTAAATAGAAAACATCATCACTAGGGATGTTAGTTTTTTTA
TCAAAACCGGTTCCAAGAGAATGCAACGGGACTTCTCACTTCGTGCTGCGGATTGCTCCA
AGTGATGGAACCAAACTCAGCCTTGTTCGTCATACTCACTCCTTTCTTGCTAACAAACGT
GACTGTGTACCTCTTCTTCTCTCCCACGCTTTTAAACGAAAGTTTCGATGGTTTAACAGA
GATTCCGACACTAGGAGCTCCATTAACCGTCACTTTGTAAACCGAGCTTGCTGTCCGGGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCG
ACGTGTCTACATTCA
   
Sequence Name 42-K017280-022-168-E04C-8409
GenBank Accession LN504285 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K017280-022-168-E04C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCCGGACAGCAAGCTCGGTT
TACAAAGTGACGGTTAATGGAGCTCCTAGTGTCGGAATCTCTGTTAAACCATCGAAACTT
TCGTTTAAAAGCGTGGGAGAGAAGAAGAGGTACACAGTCACGTTTGTTAGCAAGAAAGGA
GTGAGTATGACGAACAAGGCTGAGTTTGGTTCCATCACTTGGAGCAATCCGCAGCACGAA
GTGAGAAGTCCCGTTGCATTCTCTTGGAACCGGTTTTGATAAAAAAACTAACATCCCTAG
TGATGATGTTTTCTATTTAAGTTATCTTGATTGGGTTTAGGCTAAACCAAAGCCCATCAT
GAGCCATAAGTAGTGGGAAGTGTTCTTGTGTGGTTTGGTTTGAGTCATTGAGTGTGTAGG
TGAAAGC

Last Updated on 06.12.2018 16:11