Sequences from confirmation amplicon


Confirmation sequences for line 169A01 and hit At5g59390 are shown.
Line ID 169A01
Locus At5g59390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23957244
Primers used for PCR 08uj - 8474, show primer details
   
Sequence Name 57-K117671-0022-169-A01C-08uj
GenBank Accession LN509531 [GenBank]
Sequencing primer gene specific primer, 08uj: 5'-CAATTCTCAAAAGATGGGGTTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K117671-0022-169-A01C-08uj
CATCCCATTCCAATGTGAAATGCAGAGAGTTGTGGATGAAAACGACCATTAGCTTCGTAC
GCTGAAGAACCAGTACGGTGAAGAAGTTTATAATGAAGTGGTAAGAACAAAACTAGAGAT
TGAGGAACATAACGCTAGTGGCAGCTATGTGATTTTAGAGCTTTGGAACTATGAGGAAAA
CCGAAAGGCGACGATGGAGGAAGCAACAGATGTGATGTTGAAGATAAGGTCCAAACTTGC
TGCTATGAAGAACAAACGAAAGAGGCTTGAGGTCTAACTTAAAGAAAGAAGACAATAATT
CAAGTAACAATACCGGAGACGAGAAAGTGTTCTGTTTTTCGTTAATTTACCAGTTTAAAT
TAGTTTTGCTTCATGTCCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCACGGTTATTAT
   
Sequence Name 09-K117670-0022-169-A01C-8409
GenBank Accession LN490376 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K117670-0022-169-A01C-8409
CATTTACAATTGGACATGAAGCAAAACTAATTTAAACTGGTAAATTAACGAAAAACAGAA
CACTTTCTCGTCTCCGGTATTGTTACTTGAATTATTGTCTTCTTTCTTTAAGTTAGACCT
CAAGCCTCTTTCGTTTGTTCTTCATAGCAGCAAGTTTGGACCTTATCTTCAACATCACAT
CTGTTGCTTCCTCCATCGTCGCCTTTCGGTTTTCCTCATAGTTCCAAAGCTCTAAAATCA
CATAGCTGCCACTAGCGTTATGTTCCTCAATCTCTAGTTTTGTTCTTACCACTTCATTAT
AAACTTCTTCACCGTACTGGTTCTTCAGCGTACGAAGCTTCTCGTCGTTTTCATCCACAA
CTCTCTGCATTTCACATTTGGAATGTGGATGATATAATATGTAAAAGCAGAGGTCTTTTT
TTGCTAAAACCCCATCTTTTGGAGAATTGAAAAAANAANGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGT

Last Updated on 06.12.2018 16:11