Sequences from confirmation amplicon


Confirmation sequences for line 169C07 and hit At1g32230 are shown.
Line ID 169C07
Locus At1g32230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11612185
Primers used for PCR JK21 - 8474, show primer details
   
Sequence Name 52-K023016-022-169-C07C-8409
GenBank Accession LN507686 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K023016-022-169-C07C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTTACATTTACAATTGGTA
ATTGAAGAAGAAATTCTTAAGTCGGCGGTTCCTGAGTTTTTTTCCTCTTTCTCTAACCAA
AAATTTGCTTGGACGAAAAGCAAATCATGTGGGCTTTATTAAAGCTACAAGTCGGTCAGT
CAACNGTCGAAATNCTTATTGGGGGTGGTGACTTAATTGAAGAGGNNCGGTCAATNNACA
CGCAGAAATAGATNNATGGTCGAATNNACAATA
   
Sequence Name 53-K023016-022-169-C07C-JK21
GenBank Accession LN508017 [GenBank]
Sequencing primer gene specific primer, JK21: 5'-ATCAATCATGTTCCGTCAATACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K023016-022-169-C07C-JK21
TAATTCAACAATTTTATAATTGTTGCCCTCGTTACATTTAATTGTAATATTTTTGTTCTC
CTCATGATAATATTATTCCATTATTGTATTCGACCATATCTATCTGCGTGTATTGACCGC
CTCTTCAATTAAGTCACCACCCAATAAGATTTCGACGTTGACTGACCGACTTGTAGCTTT
AATAAAGCCCACATGATTTGCTTTTCGTCCAAGCAAATTTTTGGTTAGAGAAAGAGGAAA
AAAACTCAGGAACCGCCGACTTAAGAATTTCTTCTTCAATTACCAATTGTAAATGTAAGG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGAT
CCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTC
GACGTGTCTACATTC

Last Updated on 06.12.2018 16:11