Sequences from confirmation amplicon


Confirmation sequences for line 169C07 and hit At3g18140 are shown.
Line ID 169C07
Locus At3g18140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6214469
Primers used for PCR 08wz - 8474, show primer details
   
Sequence Name 23-K118781-0022-169-C07C-8409
GenBank Accession LN496769 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K118781-0022-169-C07C-8409
TTACATTGAATATGTCCTGATTCGGTTTGTTTCTCTCTCTCTCTCCCTTGATTTTGTGAA
TTCTGAGTAATTAATAAAACTGAGACAAATTAATGTTTGTAATTGCAGCATGTAAATAGG
CTTGAGATAACCCCAGATAAGCATTATCTAGCTGCAGCTTGCAATCCTCATATTCGACTC
TTTGATGTCAATTCCAATAGTCCTCAACCTGTAAAACTCCTCTCTTTTTTTCTCAATGTG
GTGTTTGCTTCCTTCTACCTGCTTGAGTGATCTTGATGGAGTCGTCGTGTGTATTAGGTG
ATGACTTACGATTCACACACCAACAATGTTATGGCAGTAGGATTTCAATGTGATGCAAAA
TGGATGTATTCAGGATCAGAAGATGGCACAGTTAAGATCTGGGACTTAAGGTATATCATG
TCATCTTCATCTTTAGCTAAAAGTTTATAGATTTATTCAAGTGGGAAATTCTGGG
   
Sequence Name 63-K118782-0022-169-C07C-08wz
GenBank Accession LN511092 [GenBank]
Sequencing primer gene specific primer, 08wz: 5'-CCCAGAATCCACACTTGAATAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K118782-0022-169-C07C-08wz
GATATACCTTAAGTCCCAGATCTTAACTGTGCCATCTTCTGATCCCGATTACATCCATTT
TGCATCACATTGAAATCCTACTGCCATAACATTGTTGGTGTGTGAATCGTAAGTCATCAC
CTAATACACACGACGACTCCATCAAGATCACTCAAGCAGGTAGAAGGAAGCAAACACCAC
ATTGAGAAAAAAAGAGAGGAGTTTTACAGGTTGAGGACTATTGGAATTGACATCAAAGAG
TCGAATATGAGGATTGCAAGCTGCAGCTAGATAATGCTTATCTGGGGTTATCTCAAGCCT
ATTTACATGCTGCAATTACAAACATTAATTTGTCTCAGTTTTATTAATTACTCAGAATTC
ACAAAATCAAGGGAGAGAGAGAGAGAAACAAACCGAATCAGGACATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTATATGTC

Last Updated on 06.12.2018 16:11