Sequences from confirmation amplicon


Confirmation sequences for line 169F08 and hit At1g32490 are shown.
Line ID 169F08
Locus At1g32490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11743262
Primers used for PCR 72cs - 8474, show primer details
   
Sequence Name 24-K109642-0022-169-F08C-72cs
GenBank Accession LN497031 [GenBank]
Sequencing primer gene specific primer, 72cs: 5'-AACCACTCTGGTTTCAACTCTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K109642-0022-169-F08C-72cs
CATACAGACAACCGATGAATGAAACCAATAAGTTAAACCAAAAGTAATTAAGAACAGAAA
TACAATTATGGCTAAACCTTGCAGTATCAAACATATTAATTACCTGTCGCATGTATTCCT
TGGACGTAAGTACTAGTTCGTGATATACAACCCATCTCGGCAATACCTACAACACGCATT
GAGAAAAACTTATCAGTATTCAAAGTTTTGAGATTCTGTCAAATCAGATATTTGTGGTTT
ATGTAGAGTGAGAATTCATTAACCTGAGATAAGCCCGAATTGGGATGTATGTGCACTGTC
TGGGGATGTTTCACTGTTCGGTACGATCCATTCTTCTGTAGCTTTGCAGTGTGCGGAAAA
AACCCTGAGTATATAAGTAAGAAGTTCATTAGCAGAGGCATAATTGTAAAGAATTATACA
TTTCAAATCAACAAAAAGGAGAATGAGATTGGCAATTACCGGCTACGATGGATTTTCTAA
CTGAATCCAATTCATTCAGGTTTGAGCTGATATCTATTTCCACCCTTTCGAGAAGTCCCT
CCAATTGATCACGGATATCTCTGGCTCTTTTCATGCTCCGGACCTGAACACATGTTTAAC
CCAAAACAAAGGTCAATTGGATCCACATGTGATCTGATAAACATAAAGAAACCACTGAAT
ATGAACTGAATGTAGAAATCTACATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAAATTATTTCCCATTTTTTTTTCAATTCA
AAATGT
   
Sequence Name 08-K109633-0022-169-F08C-8409
GenBank Accession LN489773 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K109633-0022-169-F08C-8409
ATTTCTACATTCAGTTCTATTCAGTTGTTTCTTTATGTTTATCAGATCACATGTGGATCC
AATTGACCTTTGTTTTGGGTTAAACATGTGTTCAGGTCCGGAGCATGAAAAGAGCCAGAG
ATATCCGTGATCAATTGGAGGGACTTCTCGAAAGGGTGGAAATAGATATCAGCTCAAACC
TGAATGAATTGGATTCAGTTAGAAAATCCATCGTAGCCGGTAATTGCCAATCTCATTCTC
CTTTTTGTTGATTTGAAATGTATAATTCTTTACAATTATGCCTCTGCTAATGAACTTCTT
ACTTATATACTCAGGGTTTTTTCCGCACACTGCAAAGCTACAGAAGAATGGATCGTACCG
AACAGTGAAACATCCCCAGACAGTGCACATACATCCCAATTCGGGCTTATCTCAGGTTAA
TGAATTCTCACTCTACATAAACCACAAATATCTGATTTGACAGAATCTCAAAACTTTGAA
TACTGATAAGTTTTTCTCAATGCGTGTTGTAGGTATTGCCGAGATGGGTTGTATATCACG
AACTAGTACTTACGTCCAAGGAATACATGCGACAGGTAATTAATATGTTTGATACTGCAA
GGTTTAGCCATAATTGTATTTCTGTTCTTAATTACTTTTGGTTTAACTTATTGGTTTCAT
TCATCGGTTTGTCTGTATGCTACTAAAACTCCCATACCCGAAAATGCAGGTAACAGAGTT
GA
Primers used for PCR 01dj - 8474, show primer details
   
Sequence Name 21-K109674-0022-169-F08C-01dj
GenBank Accession LN495700 [GenBank]
Sequencing primer gene specific primer, 01dj: 5'-AACCGATGAATGAAACCAATAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K109674-0022-169-F08C-01dj
GAATACATTATGGCTAACCTTGCAGTATCAAACATATTAATTACCTGTCGCATGTATTCC
TTGGACGTAAGTACTAGTTCGTGATATACAACCCATCTCGGCAATACCTACAACACGCAT
TGAGAAAAACTTATCAGTATTCAAAGTTTTGAGATTCTGTCAAATCAGATATTTGTGGTT
TATGTAGAGTGAGAATTCATTAACCTGAGATAAGCCCGAATTGGGATGTATGTGCACTGT
CTGGGGATGTTTCACTGTTCGGTACGATCCATTCTTCTGTAGCTTTGCAGTGTGCGGAAA
AAACCCTGAGTATATAAGTAAGAAGTTCATTAGCAGAGGCATAATTGTAAAGAATTATAC
ATTTCAAATCAACAAAAAGGAGAATGAGATTGGCAATTACCGGCTACGATGGATTTTCTA
ACTGAATCCAATTCATTCAGGTTTGAGCTGATATCTATTTCCACCCTTTCGAGAAGTCCC
TCCAATTGATCACGGATATCTCTGGCTCTTTTCATGCTCCGGACCTGAACACATGTTTAA
CCCAAAACAAAGGTCAATTGGATCCACATGTGATCTGATAAACATAAAGAAACAACTGAA
TATGAACTGAATGTAGAAATCTACATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCG
   
Sequence Name 05-K109673-0022-169-F08C-8409
GenBank Accession LN488397 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K109673-0022-169-F08C-8409
ATTTCTACATTCAGTTCTATTCAGTTGTTTCTTTATGTTTATCAGATCACATGTGGATCC
AATTGACCTTTGTTTTGGGTTAAACATGTGTTCAGGTCCGGAGCATGAAAAGAGCCAGAG
ATATCCGTGATCAATTGGAGGGACTTCTCGAAAGGGTGGAAATAGATATCAGCTCAAACC
TGAATGAATTGGATTCAGTTAGAAAATCCATCGTAGCCGGTAATTGCCAATCTCATTCTC
CTTTTTGTTGATTTGAAATGTATAATTCTTTACAATTATGCCTCTGCTAATGAACTTCTT
ACTTATATACTCAGGGTTTTTTCCGCACACTGCAAAGCTACAGAAGAATGGATCGTACCG
AACAGTGAAACATCCCCAGACAGTGCACATACATCCCAATTCGGGCTTATCTCAGGTTAA
TGAATTCTCACTCTACATAAACCACAAATATCTGATTTGACAGAATCTCAAAACTTTGAA
TACTGATAAGTTTTTCTCAATGCGTGTTGTAGGTATTGCCGAGATGGGTTGTATATCACG
AACTAGTACTTACGTCCAAGGAATACATGCGACAGGTAATTAATATGTTTGATACTGCAA
GGTTTAGCCATAATTGTATTTCTGTTCTTAATTACTTTTGGTTTAACTTATTGGTTTCT

Last Updated on 06.12.2018 16:11