Genotyping and primer details


Genotyping and primer details for 169F08 - At1g32490
Line ID 169F08
Locus At1g32490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:11743262 of line 169F08 Chr1:11743262
   
Insertion allele amplicon 8474 - 01dj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 01dj: 5'-AACCGATGAATGAAACCAATAAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:11742616
   
Insertion allele amplicon 8474 - 72cs
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 72cs: 5'-AACCACTCTGGTTTCAACTCTGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:11742550
   
wt amplicon (over InsPos) 05em - 72cs
wt amplicon start Chr1:11742550
wt amplicon end Chr1:11743543
wt amplicon size 994 bp
Locus specific primer 72cs: 5'-AACCACTCTGGTTTCAACTCTGTT-3'
2nd locus specific primer 05em: 5'-CTATGTTGTCAATTGGTGGCTCTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11