Sequences from confirmation amplicon


Confirmation sequences for line 169H04 and hit At4g18205 are shown.
Line ID 169H04
Locus At4g18205 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10073858
Primers used for PCR UF16 - 8474, show primer details
   
Sequence Name 26-K021852-022-169-H04C-UF16
GenBank Accession LN497731 [GenBank]
Sequencing primer gene specific primer, UF16: 5'-CGTATACTCTAGGAGCTTGGCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K021852-022-169-H04C-UF16
TACGTCATAGACACTGAGGAAAAGAGTTCTCTTAGAGCCGCCATTCAAAAGTTTTAAGAA
TTTTAAGTCAAGTGTAAGCAAATAAGAAAGGTCAATGGTCAGCATGAGACTACTAAATTA
TGATACTGCTGTCATCTATCACCTGACCCATCTTTATTATTATACACAAATTAGTAAAAG
TCTGCCAAGTTTGATTAGTGCAACTTGTATCTGGGGTATTTAGTTACTATAATAAATATC
ATTTTGTGTATAAAGAAAGGTCTCTTCTGTATCTAAAAGACATATCAAACGAAAGAAATC
AATAAACACAATATTCTTTCTTCCAAATTTTCTCATTTCTCATGGGCATAATTTTATTTG
TATTTTATTTATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAA
AACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATT
CGGAAATCTTTATTTCGACGTGTCTACT
   
Sequence Name 25-K021852-022-169-H04C-8409
GenBank Accession LN497253 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K021852-022-169-H04C-8409
AGCCATTTACATTGAATATATCCTGGATAAATAAAATACAAATAAAATTATGCCCATGAG
AAATGAGAAAATTTGGAAGAAAGAATATTGTGTTTATTGATTTCTTTCGTTTGATATGTC
TTTTAGATACAGAAGAGACCTTTCTTTATACACAAAATGATATTTATTATAGTAACTAAA
TACCCCAGATACAAGTTGCACTAATCAAACTTGGCAGACTTTTACTAATTTGTGTATAAT
AATAAAGATGGGTCAGGTGATAGATGACAGCAGTATCATAATTTAGTAGTCTCATGCTGA
CCATTGACCTTTCTTATTTGCTTACACTTGACTTAAAATTCTTAAAACTTTTGAATGGCG
GCTCTAAGAGAACTCTTTTTCCTCAGTGTCTATTGACGTTAAATCAGAAGTCAACAAAGA
TACAAGAAGCCAAGCTCC

Last Updated on 06.12.2018 16:11