Sequences from confirmation amplicon


Confirmation sequences for line 169H04 and hit At5g43930 are shown.
Line ID 169H04
Locus At5g43930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17679518
Primers used for PCR 21kn - 8474, show primer details
   
Sequence Name 47-K113595-0022-169-H04C-21kn
GenBank Accession LN506254 [GenBank]
Sequencing primer gene specific primer, 21kn: 5'-CAAATAGATCAGATTTCCCTGAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K113595-0022-169-H04C-21kn
TTTAGGATCGAGTTCCTGGGAGTTACCTTTTCTACAAGGATGGTTGATGGCTCAAGGTCA
TGGTGTTGCTAATCCAGTGGTTACTCCTACTGGCAGTAGTAATCATGGCATCTCAGCTCC
ATCCTCAACAGCCAGTCTGGAGGCTGCAGTAGCGTTATTAGAAATCCCGAGTGGTGTTAA
CTTACATGCGGTGTCTAGAAGAGGCGGGGCTCAGGAACAAACTTCACAACCCCAATTCTC
GAGAACTGGATTACCGGAAGGTGTATCTTCTCGTAACACTCAACATGGAAGTGATGCTCA
ACCTGTAGTAAATAGGGTCCAGTCTGAGCTTGCTACCTCAATTGCTGCTTCGGCTGCAGC
AGCTGCTGCTGCAGAATTACCTTGTACTGTCAAACTCAGAATGTGGTCGCATGACATCAA
AGACCCATATGCACAACTGAAATCCGACAGATGTCTATTTACAATACAGGATATATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGC
A
   
Sequence Name 05-K113594-0022-169-H04C-8409
GenBank Accession LN488435 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K113594-0022-169-H04C-8409
CATTTCATTGAATATATCCTGTATTGTAAATAGACATCTGTCGGATTTCAGTTGTGCATA
TGGGTCTTTGATGTCATGCGACCACATTCTGAGTTTGACAGTACAAGGTAATTCTGCAGC
AGCAGCTGCTGCAGCCGAAGCAGCAATTGAGGTAGCAAGCTCAGACTGGACCCTATTTAC
TACAGGTTGAGCATCACTTCCATGTTGAGTGTTACGAGAAGATACACCTTCCGGTAATCC
AGTTCTCGAGAATTGGGGTTGTGAAGTTTGTTCCTGAGCCCCGCCTCTTCTAGACACCGC
ATGTAAGTTAACACCACTCGGGATTTCTAATAACGCTACTGCAGCCTCCAGACTGGCTGT
TGAGGATGGAGCTGAGATGCCATGATTACTACTGCCAGTAGGAGTAACCACTGGATTAGC
AACACCATGACCTTGAGCCATCAACCATCCTTGTAGAAAAGGTAACTCCCAGGAAACTCG
ATCCCTAAAGTGAAATAATTGCCGAATCTGTCCAAGCTCAGGGAAATCTGATCTATTTG

Last Updated on 06.12.2018 16:11