Sequences from confirmation amplicon


Confirmation sequences for line 170A10 and hit At3g61630 are shown.
Line ID 170A10
Locus At3g61630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22806295
Primers used for PCR WD03 - 8474, show primer details
   
Sequence Name 15-K017176-022-170-A10C-WD03
GenBank Accession LN492745 [GenBank]
Sequencing primer gene specific primer, WD03: 5'-CGATTCATCTCAAAGGACCTAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K017176-022-170-A10C-WD03
CGAATTTCCTAACTCCGCCGACGCCAACGCCGGTTATCGATCTCCAAACGGTTTCCGCCT
GCGATTACGGTAGAGATTCTCGGCAGAGCCTTCATTCACCGACCTCTGTTCTAAGATTCA
ACGTCAACGAGGAAACAGAGCATGAGATTGAAGCGATCGAGCTATCTCCGGAGAGAAAGT
CGACGGTTATAAAAGAAGAAGAAGAATCGTCGGCGGGTTTGGTGTTCCCGGATCCGTATC
TGTTACCGGATTTATCTCTCGCCGGCGAATGTTTTTGGGATACCGAAATTGCCCCTGACC
TTTTGTTTCTCGATGAAGAAACCAAAATCCAATCAACGTTGTTACCAAACACAGAGGTTT
CGAAACAAGGAGAAAACGAAACTGAAGATTTCGAGTTTGGTTTGATTGATGATTTCGAGT
CTTCTCCATGGGATGTGGATCATTTCTTCGACCATCATCATCACTCTTTCGATTAAAAAT
CTCTTCTTTTTTGGGGAAATTTTTGTGTGGTGATGATGACGTGGATACATCGTGACCGTC
GGCTTACCTTATATTGTATGATGGATCAATTGAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGAT
AAAACGACAAATT
   
Sequence Name 19-K017176-022-170-A10C-8409
GenBank Accession LN494548 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K017176-022-170-A10C-8409
TAGATTCCCGGACATGAAGCCATTTCAATTGATCCATCATACAATATAAGGTAAGCCGAC
GGTCACGATGTATCCACGTCATCATCACCACACAAAAATTTCCCCAAAAAAGAAGAGATT
TTTAATCGAAAGAGTGATGATGATGGTCGAAGAAATGATCCACATCCCATGGAGAAGACT
CGAAATCATCAATCAAACCAAACTCGAAATCTTCAGTTTCGTTTTCTCCTTGTTTCGAAA
CCTCTGTGTTTGGTAACAACGTTGATTGGATTTTGGTTTCTTCATCGAGAAACAAAAGGT
CAGGGGCAATTTCGGTATCCCAAAAACATTCGCCGGCGAGAGATAAATCCGGTAACAGAT
ACGGATCCGGGAACACCAAACCCGCCGACGATTCTTCTTCTTCTTTTATAACCGTCGACT
TTCTCTCCGGAGATAGCTCGATCGCTTCAATCTCATGCTCTGTTTCCTCGTTGACGTTGA
ATCTTAGAACAGAGGTCGGTGAATGAAGGCTCTGCCGAGAATCTCTACCGTAATCGCAGG
CGGAAACCGTTTGGAGATCGATAACCGGCGTTGGCGTCGGCGGAGTTAGGAAATTCGTGA
GCGCTTTAGGTCCTTT

Last Updated on 06.12.2018 16:11