Genotyping and primer details

Genotyping and primer details for 170A10 - At3g61630
Line ID 170A10
Locus At3g61630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22806295 of line 170A10 Chr3:22806295
Insertion allele amplicon 8474 - WD03
Locus specific primer WD03: 5'-CGATTCATCTCAAAGGACCTAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22805716
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11