Sequences from confirmation amplicon


Confirmation sequences for line 171B03 and hit At5g61000 are shown.
Line ID 171B03
Locus At5g61000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24549806
Primers used for PCR 10kk - 8474, show primer details
   
Sequence Name 68-K113595-0022-171-B03C-10kk
GenBank Accession LN512054 [GenBank]
Sequencing primer gene specific primer, 10kk: 5'-ATCACAAATTACAGTGGTCGATGA-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K113595-0022-171-B03C-10kk
ACTCTCTAGACTCTACAGCTGTAAACACAAAATAGATCGCTTGTTTTTATTTGTTAATGA
TACCGAAATGGATGGCTTGTAAACAATGTAAAGAACAAATTTTAAGCAAAACCCTAATTT
TATAAATTGCAGAAAGGCTTATATTATACAAGGAAAAGTTTTGAGACTTTTTTAGGCCAA
TAGAGAAAAAAAAAGAAGTAAAAGCTACTACTGAGATGTTTTGTTCTTGTTGGAGATGTC
TTGAAGCAACAATCTTGTTTCAGCAGCAAAATCAAGAGGAGCTACTCCTTTTACAGTTAC
CCTCTGTCTCTTCTCACCGTTATACTCATTCTTCTTCAGGATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 26-K113594-0022-171-B03C-8409
GenBank Accession LN498013 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K113594-0022-171-B03C-8409
TTGAATATATCCTGAAGAAGAATGAGTATAACGGTGAGAAGAGACAGAGGGTAACTGTAA
AAGGAGTAGCTCCTCTTGATTTTGCTGCTGAAACAAGATTGTTGCTTCAAGACATCTCCA
ACAAGAACAAAACATCTCAGTAGTAGCTTTTACTTCTTTTTTTTTCTCTATTGGCCTAAA
AAAGTCTCAAAACTTTTCCTTGTATAATATAAGCCTTTCTGCAATTTATAAAATTAGGGT
TTTGCTTAAAATTTGTTCTTTACATTGTTTACAAGCCATCCATTTCGGTATCATTAACAA
ATAAAAACAAGCGATCTATTTTGTGTTTACAGCTGTAGAGTCATAGAGAGTTGTGTAATT
TGTGAAGATAACTTTTCATCGACCACTGTAATTTGTGAT

Last Updated on 06.12.2018 16:11