Sequences from confirmation amplicon


Confirmation sequences for line 171B04 and hit At1g47990 are shown.
Line ID 171B04
Locus At1g47990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17700108
Primers used for PCR AP02 - 8474, show primer details
   
Sequence Name 35-K026964-022-171-B04C-8409
GenBank Accession LN501689 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K026964-022-171-B04C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAAGCCATTGGTAATTACTCTTTCTTTT
TCATTACTCAGTGACACAGATTGGTCAGAAAGATTGGCTTCACCAAAGAATTGATCGGAC
GGTGGATAATGATTCACTCTCAGAACGGAGTCACTATCGACGGAGCTGATTAACCTACTG
AAACTGTGAGGTGGGACATGAAGTCCCTCAGCCGTCAGATCTAAGATCTCACGAGCCAAC
TGCTTAACTGCTTCTATGTAACAATTCACTGCCGAGCTGTCAAACATATACGTCACACAT
CAGCACGAGATTCATTTATTTTTGG
   
Sequence Name 39-K026964-022-171-B04C-AP02
GenBank Accession LN503226 [GenBank]
Sequencing primer gene specific primer, AP02: 5'-ACCAAAAATAAATGAATCTCGTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K026964-022-171-B04C-AP02
TGATGTGTGACGTATATGTTTGACAGCTCGGCAGTGAATTGTTACATAGAAGCAGTTAAG
CAGTTGGCTCGTGAGATCTTAGATCTGACGGCTGAGGGACTTCATGTCCCACCTCACAGT
TTCAGTAGGTTAATCAGCTCCGTCGATAGTGACTCCGTTCTGAGAGTGAATCATTATCCA
CCGTCCGATCAATTCTTTGGTGAAGCCAATCTTTCTGACCAATCTGTGTCACTGAGTAAT
GAAAAAGAAAGAGTAATTACCAATGGCTTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTT
TGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATT
CTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11