Sequences from confirmation amplicon


Confirmation sequences for line 171C11 and hit At5g16030 are shown.
Line ID 171C11
Locus At5g16030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5239152
Primers used for PCR 04mh - 8474, show primer details
   
Sequence Name 47-K114263-0022-171-C11C-04mh
GenBank Accession LN506268 [GenBank]
Sequencing primer gene specific primer, 04mh: 5'-ACAATAACAGGGAATGGTTAGCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K114263-0022-171-C11C-04mh
TATATCTGCAATCCATTGTCTGGAGAAGTACCAATGGAGTGTTTGTCTGCTAAAACATTA
AGTGCAAGATCCTTCAGAAACTTATCAACCATGTCTGCTCCTCTTCACTTCCCTAGTCCC
AATCCATTGATGAATAATATTGCTCAAAACAAACCAAATAATAATCCTAACGTTAGAGTC
ATTCACGAGGATCTTTATGCTCCTGATCCTGAACTTCTTGCTCTAGGTGATTGACTTTAA
CTCTGTCCCATCTTTTAGATTTTTTTTTAGGTTTCTTGAAGTTTGTTCTTGATTGGGTTT
TGCTGATCAAAAGTAAATTATGGGGGGGTGTTTTTAGCGGAAAAGAAAGTTGTGGGGATG
AAGCGAGATGTGGGCATACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGAAGTCCGCAACATTA
   
Sequence Name 04-K114262-0022-171-C11C-8409
GenBank Accession LN488009 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K114262-0022-171-C11C-8409
GAATATATCCTGTATGCCCACATCTCGCTTCATCCCCACAACTTTCTTTTCCGCTAAAAA
CACCCCCCCATAATTTACTTTTGATCAGCAAAACCCAATCAAGAACAAACTTCAAGAAAC
CTAAAAAAAAATCTAAAAGATGGGACAGAGTTAAAGTCAATCACCTAGAGCAAGAAGTTC
AGGATCAGGAGCATAAAGATCCTCGTGAATGACTCTAACGTTAGGATTATTATTTGGTTT
GTTTTGAGCAATATTATTCATCAATGGATTGGGACTAGGGAAGTGAAGAGGAGCAGACAT
GGTTGATAAGTTTCTGAAGGATCTTGCACTTAATGTTTTAGCAGACAAACACTCCATTGG
TACTTCTCCAGACAATGGATTGCAGATATATTTCTCTGCTTCGTTCCACTTCGAATTCAA
TGCAAACCCTTCTTGTGCTAACCATTCCTGNTTATT

Last Updated on 06.12.2018 16:11