Sequences from confirmation amplicon


Confirmation sequences for line 172B09 and hit At5g07580 are shown.
Line ID 172B09
Locus At5g07580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2400205
Primers used for PCR MZ02 - 8474, show primer details
   
Sequence Name 51-K015907-022-172-B09C-8409
GenBank Accession LN507336 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K015907-022-172-B09C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTCCAATTGTAAATGA
AGCCATATGGACCATCATATTTAGGGTAGCCATTTAAAAGCCGTGCTCAACTTCCCTCTT
GACGCCGGGAAATATGAAGCTCCAGCGAATTCAGGAAGGAAAAGGAAGAGAAGTGATGTG
CATGAAGAGCTTCAAAGAACTCAGAGCAATTCATCTTCATCTTCCTGTGATGCATTTTAG
CATATTAAGAGTGTGAGCAGTTTCCTTAAGTTGTATAAAGTAATTGTACAGAGGAAACGA
ATTGTGTAGGTTTAGTGTGCTTGCAAGTTGCAACAAATGTGTATGGATGTTCTGTTTCTT
CATGTCCCTAAGATTTAGAAACATCTTCTTATTTCCAAGAAAGTTATTCAGTTTCCAGGT
CTCTAAACAA
   
Sequence Name 52-K015907-022-172-B09C-MZ02
GenBank Accession LN507657 [GenBank]
Sequencing primer gene specific primer, MZ02: 5'-GGTTGTTTAGAGACCTGGAAACTG-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K015907-022-172-B09C-mz02
CTTTCTTGGAAATAAGAAGATGTTTCTAAATCTTAGGGACATGAAGAAACAGAACATCCA
TACACATTTGTTGCAACTTGCAAGCACACTAAACCTACACAATTCGTTTCCTCTGTACAA
TTACTTTATACAACTTAAGGAAACTGCTCACACTCTTAATATGCTAAAATGCATCACAGG
AAGATGAAGATGAATTGCTCTGAGTTCTTTGAAGCTCTTCATGCACATCACTTCTCTTCC
TTTTCCTTCCTGAATTCGCTGGAGCTTCATATTTCCCGGCGTCAAGAGGGAAGTTGAGCA
CGGCTTTTAAATGGCTACCCTAAATATGATGGTCCATA

Last Updated on 06.12.2018 16:11