Sequences from confirmation amplicon


Confirmation sequences for line 172F11 and hit At2g47000 are shown.
Line ID 172F11
Locus At2g47000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19311183
Primers used for PCR MB01 - 8474, show primer details
   
Sequence Name 54-K023016-022-172-F11C-8409
GenBank Accession LN508347 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K023016-022-172-F11C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAAAACAGAGTCGAAGG
TTGTTTTGCCATCATCGACAAGGCGTGCTCCAACGTAGAAACTGGCAGCGTAAGAAGAGA
AGAGGACGAAGAAAGAGAAGCCGAAACCAATACCACTGACTATGCCTTGACGAATCCCAT
TCTTCATTGGACCTTCACATTTCTTTGAGTACATGTTCATCACCTTGTCTTCTGCACAGA
ATGAAGCAACGGTTCTTATGCTCCCTACCGCGTCATTCGCTACTTGGCTTGCTTCCCCGT
ACATTTTCTGCCAATGATTTAAAGTAACGTTAATCAAATGATTGAGAGAATCTAGTTGGT
ATATGTATGAAAAGTAGTAACTAGTTTACCTATCTTAGCAAAAAAAAAAAAAAAAAAACC
TTTTTTCCTTTTGCTTCCGCGGGAAACCTTTTCGGGAATTTCTTAAAAAAAAACCCTTTG
GGGGGAAAAAGGGGGACCTTCCGGGGGCCCCCA
   
Sequence Name 55-K023016-022-172-F11C-MB01
GenBank Accession LN508677 [GenBank]
Sequencing primer gene specific primer, MB01: 5'-GTCTAGTTGGAGACTCTTTGGCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K023016-022-172-F11C-MB01
GAATCTTTCATCTATATTGGCTGGTTTGATCATTGCATTTTTGGCTTGTTGGCAATTAGC
TTTCGTGGTCCTCGCGATGCTCCCGCTTATCGCACTCAATGGATTTCTTTATATGAAGTT
CATGAAAGGTTTCAGCGCTGATGCAAAGGTAAACTAGTTTTTTTTTTTTTTTTTTTGGCA
AAAAAGGGAAAACTATTTCCTCTTTTTCAAAAAAAACCCAACAAATTTCCCCCAATCTTT
TGATAAACGTTCTTTAAAACCTTGGGAAAAAAATGTGGGGGAAACCACCCCAATTACCAA
AGGCCCCGGGGGGGAAAAAAAAAACCTTTTTTTTTTTTTGGGAAAAAAAAAGGGGGATAA
ACTTTTCCCCAAAAAATTGGGAGGGCCCAAAAAAAAAGGGGTTTCCCCAGGGCAAACCCG
GGGGTTTGGGTTTCGGTTTTTTTTTTTTCGCCCTTTTTTTTTTTAACGGGGCCGTTT

Last Updated on 06.12.2018 16:11