Sequences from confirmation amplicon


Confirmation sequences for line 172G01 and hit At5g06660 are shown.
Line ID 172G01
Locus At5g06660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2047277
Primers used for PCR BL53 - 8474, show primer details
   
Sequence Name 49-K104987-0022-172-G01C-BL53
GenBank Accession LN506835 [GenBank]
Sequencing primer gene specific primer, BL53: 5'-TTTGAAAGTGTTTCTCATCAAACG-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K104987-0022-172-G01C-BL53
CTATGAAAATTTCAGAGTAACAACGAGCTAAGAATCCAGAATCCAATTCATCATCAGATC
AAACCTGATCAATTGGTCTTTGGATCAGGCATTGGGAACAAACCACCAGCACCAGCAGCT
CCTCTAGGTGGAGAGAAACCCAAGAACTTCTGCAGATTAGTCCTTATACTAATAGAACAA
AGCAGATAAAGAAAAGCCATGGAACAATCAGTGGAATCATCGCCTTTCAATCCTCTATGA
CTCATGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCG
   
Sequence Name 33-K104988-0022-172-G01C-8409
GenBank Accession LN500988 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K104988-0022-172-G01C-8409
ATTTACAATTGAATATATCATGAGTCATAGAGGATTGAAAGGCGATGATTCCACTGATTG
TTCCATGGCTTTTCTTTATCTGCTTTGTTCTATTAGTATAAGGACTAATCTGCAGAAGTT
CTTGGGTTTCTCTCCACCTAGAGGAGCTGCTGGTGCTGGTGGTTTGTTCCCAATGCCTGA
TCCAAAGACCAATTGATCAGGTTTGATCTGATGATGAATTGGATTCTGGATTCTTAGCTC
GTTGTTACTCTGAAATTTTCATAGATAACTTCAATGCTCTGATGTAGTGGTGTTGTTTTC
AGTTCGTTTGATGAGAACANCTTTCAA

Last Updated on 06.12.2018 16:11