Sequences from confirmation amplicon


Confirmation sequences for line 172G10 and hit At2g02060 are shown.
Line ID 172G10
Locus At2g02060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:496712
Primers used for PCR 42pm - 8474, show primer details
   
Sequence Name 54-K115064-0022-172-G10C-42pm
GenBank Accession LN508572 [GenBank]
Sequencing primer gene specific primer, 42pm: 5'-TGATATTTGGAAAAAGAATGGGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K115064-0022-172-G10C-42pm
ATAATAATGGATGGAATTTATTATTTTACTGTATCTGTTTGTAGTATGTACTATTGGTCT
AGTGTCATGATTCATAAGACAAATGTCATAAATACATTTATATATTTTTCAAGAGTAATA
GTGACAGTTTGAGCAATGATACAATTGTATGACATGTATATTTTTCAAGAGTAATAGTGA
CATTTGTATTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 22-K115063-0022-172-G10C-8409
GenBank Accession LN496236 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K115063-0022-172-G10C-8409
TATATCCTGAATACAATGTCACTATTACTCTTGAAAAATATACATGTCATACAATTGTAT
CATTGCTCAAACTGTCACTATTACTCTTGAAAAATATATAAATGTATTTATGACATTTGT
CTTATGAATCATGACACTAGACCAATAGTACATACTACAAACAGATACAGTAAAATAATT
AAATTCCATCCATTATTATGTGTTTGATGTTAACAGTTCAAATTAAAATCAACGGTCCCA
TTCTTTTTNCAAATATC

Last Updated on 06.12.2018 16:11