Sequences from confirmation amplicon


Confirmation sequences for line 174D04 and hit At4g30800 are shown.
Line ID 174D04
Locus At4g30800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15002106
Primers used for PCR 12fh - 8474, show primer details
   
Sequence Name 20-K110985-0022-174-D04C-12fh
GenBank Accession LN495260 [GenBank]
Sequencing primer gene specific primer, 12fh: 5'-AATCTGATTTTAGAGTAATGCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K110985-0022-174-D04C-12fh
AGATTGGTGGTATTTGCCAATTTTAGGAGCTTTGGGGAAATTGTTTGACATAGCTATGTT
TTTTCTTGATTTTATTTGCAGGAACATACATTGACCAGAAATGTCCCTTTACTGGTACGG
TTTCAATCAGGGGTCGCATCTTGTCTGGAACTTGTCACAGTGCAAAGATGCAGAGGACGA
TCATTGTTCGTAGGGACTATCTTCATTTTGTGAAGAAGTATCGAAGGTAAAAGGATCGGT
TTTGTTATTGTGTTCAAATTCTCTCAGCTTATTTGAAACCCTCCAGCTAAATGCTTAATC
CTACCAGATATGAGAAAAGACACTCAAACATTCCTAAAATGATATATGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
ACGTTATTA
   
Sequence Name 04-K110984-0022-174-D04C-8409
GenBank Accession LN487972 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K110984-0022-174-D04C-8409
TCGGACTGAGCCTTTCATTGAATATATCCATATATCATTTTAGGAATGTTTGAGTGTCTT
TTCTCATATCTGGTAGGATTAAGCATTTAGCTGGAGGGTTTCAAATAAGCTGAGAGAATT
TGAACACAATAACAAAACCGATCCTTTTACCTTCGATACTTCTTCACAAAATGAAGATAG
TCCCTACGAACAATGATCGTCCTCTGCATCTTTGCACTGTGACAAGTTCCAGACAAGATG
CGACCCCTGATTGAAACCGTACCAGTAAAGGGACATTTCTGGTCAATGTATGTTCCTGCA
AATAAAATCAAGAAAAAACATAGCTATGTCAAACAATTTCCCCAAAGCTCCTAAAATTGG
GCAAATACCACCAATCTACTACCCAAGTCTTAGATCAATGAGAATACTAACAAAGCGGCA
TTACTCTAAAATCAGAT

Last Updated on 06.12.2018 16:11