Sequences from confirmation amplicon


Confirmation sequences for line 174D05 and hit At4g21490 are shown.
Line ID 174D05
Locus At4g21490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11439051
Primers used for PCR JZ11 - 8474, show primer details
   
Sequence Name 73-K103592-0022-174-D05C-JZ11
GenBank Accession LN512880 [GenBank]
Sequencing primer gene specific primer, JZ11: 5'-TCAACATCAAACTCTTTCTTCCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K103592-0022-174-D05C-JZ11
TATTATATCTTGCNATGTTGCGAATAGGTTCAACAACGCTGCGAGCTTCAACAGTTCCAC
AAGTAACACTAGGTAACAAGGGAGTGAAAGCAAAGTAGTTTCGAGGAGATATAACCTGAA
CCTCATAAGAAGAGTTATTCAAAGTCTTCAAAAAACTTGCTCCAGCCCATCCAGTTCCGA
GCAGCACTACTTTTCTCTTCCTGGTTTTAGTTTCAACACCATTGTTGCTATATGATGGAT
TTGCCTCAGAGTAAACTATAAGTCCCCCACCACTATGAAACAAGAAATAATAATTAGAAC
AGAACATAAGATCTAAAACAGCCATCTATATTTTTGATTATGAATATCATCAGCTCTGCT
CTCGTAAACACAGATTTCGATCTAACGTATATGACAAGTAAACATAACAAGACTCTGTTG
CATACACGGGAAAACAAAATTTTGCGAAGGAAAAGTCTCATGACGCAGCAACAATATATC
CAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTC
   
Sequence Name 67-K103593-0022-174-D05C-8409
GenBank Accession LN511796 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K103593-0022-174-D05C-8409
ATTTACAATTGGATATTAATAGGCACGTGTCCGAGAATGTTACGACTCATCCGTGTTTCG
AGGGAAGAAAGAGTTTGATGTTGAAACANAGTCTTGTTATGTTTACTTGTCATATACGTT
AGATCGAAATCTGTGTTTACGAGAGCAGAGCTGATGATATTCATAATCAAAAATATAGAT
GGCTGTTTTAGATCTTATGTTCTGTTCTAATTATTATTTCTTGTTTCATAGTGGTGGGGG
ACTTATAGTTTACTCTGAGGCAAATCCATCATATAGCAACAATGGTGTTGAAACTAAAAC
CAGGAAGAGAAAAGTAGTGCTGCTCGGAACTGGATGGGCTGGAGCAAGTTTTTTGAAGAC
TTTGAATAACTCTTCTTATGAGGTTCAGGTTATATCTCCTCGAAACTACTTTGCTTTCAC
TCCCTTGTTACCTAGTGTTACTTGTGGAACTGTTGAAGCTCGCAGCGTTGTTGAACCTAT
TCGCAACATTGCAAGAAAGGTAAGTACTACACTCTTAATCCCTCCTTATAATGGCTGCCA
TACAAAAACTAAAGCCACCTTTGTCTTGTGTCTTAACTAGCAGAATGTTGAGATGTCTTT
CTTGGAGGCAGAATGTTTCAAAATTGATCCAGGAAGCAAGAAAGTTTACTGCCGATCAAA
ACAAGGCGTGAATTCGAAAGGGAAGAAAGAGTTGGATGT

Last Updated on 06.12.2018 16:11