Sequences from confirmation amplicon


Confirmation sequences for line 174H02 and hit At2g24870 are shown.
Line ID 174H02
Locus At2g24870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10588705
Primers used for PCR 69pq - 8474, show primer details
   
Sequence Name 44-K115187-0022-174-H02C-69pq
GenBank Accession LN505284 [GenBank]
Sequencing primer gene specific primer, 69pq: 5'-ATGCAAATCGTCATTTTCTCTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K115187-0022-174-H02C-69pq
GTGAATCGGAGGTTTAATTTGTCTTTGGCTTATTTTTATTTTATTTTATTTTTCTCGCAT
ACCAAATTGGAAGACAACAGGAGGAGGAGAAAAGTCTAGGAATAACCCAATTGGGTTATT
GGACCCTGGGCCCATTAAAGTTGGATCTTTTTGTTTGCTAAAATGATTGATTCAATGATT
TAAACCAACTCATTATCTCCTCTACAATTCCTTGGATTCTTCCTACAGTCAAAACAAAAT
CCAGATTGCTTGTTTGTTTTTGGTTTGCTTATATGATTGAGAAAATAATTTTTTTCTATG
TATAAATTTACTAGTTTTAAGATGATAATATAACATTTTTTTTTGTTCTTACATAGATTG
TTAAATATTTTTAATTTAACCAATCATACACTTTTATGCATATTAATCGATTTTCCAATA
TGTTTGCCGATTAACGCGTAAATGATTTTATGCATATTTTTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 08-K115186-0022-174-H02C-8409
GenBank Accession LN489853 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K115186-0022-174-H02C-8409
TGAAAATATGCATAAAATCATTTACGCGTTAATCGGCAAACATATTGGAAAATCGATTAA
TATGCATAAAAGTGTATGATTGGTTAAATTAAAAATATTTAACAATCTATGTAAGAACAA
AAAAAAATGTTATATTATCATCTTAAAACTAGTAAATTTATACATAGAAAAAAATTATTT
TCTCAATCATATAAGCAAACCAAAAACAAACAAGCAATCTGGATTTTGTTTTGACTGTAG
GAAGAATCCAAGGAATTGTAGAGGAGATAATGAGTTGGTTTAAATCATTGAATCAATCAT
TTTAGCAAACAAAAAGATCCAACTTTAATGGGCCCAGGGTCCAATAACCCAATTGGGTTA
TTCCTAGACTTTTCTCCTCCTCCTGTTGTCTTCCAATTTGGTATGCGAGAAAAATAAAAT
AAAATAAAAATAAGCCAAAGACAAATTTAAACCTCCGATTTCAACCTTCTCGGCGAAGAA
GAAGAAGAAGAAGAGAAAAT

Last Updated on 06.12.2018 16:11