SimpleSearch - Line and FST details
Line specific information
| Line ID | 174H02 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N416694) |
| Segregation Analysis | 50:46:29 |
| Confirmed for Hit | At2g24870 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | 63980, 94965 |
| Note |
Gene hit At5g15685
| Sequence (A. th genome BLAST matches underlined) | >16-K013516-022-174-H02-8409 NNNNNTTTGATCCATGTAGCATTTCCCGTACATGAANGCCTTTACAATTGAATATGTCCT TTCTTCATTGCTGCCAAGACCAACATAAATCCGGATAGAAGACCTCATTCTCGTTCTGCA GTCCCTAGTGGCTATCAACAATTGCAGATTGAACCTTTGTCTGTACTCGATATGGCAGCC ACATATTCTCGAAGGTGTGCAAACTGTTGGGCATATTCTTCTTTCAGCCACTTAAGAGCT ATGGGATCCTCCCTTCATCGAGGANACTNCTCGCGGGGGAGGCCTCGTCAGCGGGGGCCT TGTCTGTATCGCTGTGATGGCATTTGTCTTAGCCTTTTTCCTTTTCCACTATCTTGACAT AGACTGACTGATAGCCCGCGCAATGGACTCCGAGGAGGCCGACGGATGGTCCCTTTTGTT CAAAAGGGTCTCCGGCGATGTGCTCTTGTGGAACTGTCTCTTTGATAGTCTCGGAGTAAA CAAGCGCGTC |
| GenBank Accession | AL770798 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr5:5112907 - go to primer design |
| BLAST e Value | 4e-38 |
| Hit Clone Code (BAC ID) | F14F8 |
| Hit Gene Code | At5g15685 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | mutator transposase MUDRA protein |
| Insertion Classification | CDSi |
| Confirmation Status | unknown |
Gene hit At4g30340
| Sequence (A. th genome BLAST matches underlined) | >16-K013531-022-174-H02-8409 AATTGATCACTGTAGACTTCCCTTACATGAAGCACTTTACAATTGAATATTTACTCTAGA TACATATTCGAGCTGGCATAACCACAGCTGTTAGTCACAAAATGATGAAATTAGTGGGAA ACCGCCTGAGTGGAATAGATGAGATGGTATTGCTACCTCCTCAGAAACTGTTGAGGCTCG AATAATGATGAGGATACGAACATAAGATACAACCTATGGGATCCTCCCTATATGTGAANC NNGATTNGGGAGTTTACTTTCTTTTTGACCTCACTATGGGATCCTCCCTATAGGGAGACT TCACGGAACTGCCGAGGAGATAATGAGCGGGTTCAAATTGGTGTTTCAATATTTTGCGCG GACAAAAGGATCTAACTCCAATGGGCCCAGGGACCAATATCCCAGTTGGGTTATT |
| GenBank Accession | AL770799 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr4:14840635 - go to primer design |
| BLAST e Value | 7e-06 |
| Hit Clone Code (BAC ID) | F17I23 |
| Hit Gene Code | At4g30340 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | diacylglycerol kinase 7 |
| Insertion Classification | CDSi |
| Confirmation Status | failed, line belongs to a contamination group, please check line 174H01 |
Gene hit At2g24870
| Sequence (A. th genome BLAST matches underlined) | >16-K013531-022-174-H02-8409 AATTGATCACTGTAGACTTCCCTTACATGAAGCACTTTACAATTGAATATTTACTCTAGA TACATATTCGAGCTGGCATAACCACAGCTGTTAGTCACAAAATGATGAAATTAGTGGGAA ACCGCCTGAGTGGAATAGATGAGATGGTATTGCTACCTCCTCAGAAACTGTTGAGGCTCG AATAATGATGAGGATACGAACATAAGATACAACCTATGGGATCCTCCCTATATGTGAANC NNGATTNGGGAGTTTACTTTCTTTTTGACCTCACTATGGGATCCTCCCTATAGGGAGACT TCACGGAACTGCCGAGGAGATAATGAGCGGGTTCAAATTGGTGTTTCAATATTTTGCGCG GACAAAAGGATCTAACTCCAATGGGCCCAGGGACCAATATCCCAGTTGGGTTATT |
| GenBank Accession | AL770799 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr2:10588733 - go to primer design |
| BLAST e Value | 3e-05 |
| Hit Clone Code (BAC ID) | F27C12 |
| Hit Gene Code | At2g24870 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Plant self-incompatibility protein S1 family |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
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Last Updated on 10.06.2021 13:37 |
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