Sequences from confirmation amplicon


Confirmation sequences for line 175D03 and hit At2g34840 are shown.
Line ID 175D03
Locus At2g34840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14703375
Primers used for PCR 75cs - 8474, show primer details
   
Sequence Name 25-K109642-0022-175-D03C-75cs
GenBank Accession LN497485 [GenBank]
Sequencing primer gene specific primer, 75cs: 5'-CCTCGCTATGTATCTATCGACTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K109642-0022-175-D03C-75cs
GAAACTGTTGTAAGAGATTCAAATGCTTATATGGAACGATTGTTATTGGTTCAGGAATCA
ACAATTTCAAGCTTGAAGGAATGGTTAGCAGATTCAACAATAGGAAACAATGATACATTA
CGATTGATTGCTGGTATTATTTTCATGCATGAAGAAGATTATAATGAAACTTTGAAGCAT
ACTCATGCTGGAGGAACTATGGATTTGTAAGATTTTTTCGATTTTTATTTGGTGGATTGA
GTATTTATACTGTGTTTTAAAACATGTTCTTAGATGGCTCATTGCTATTGTTTTTCTAGG
TATGCCTTGAATGTTCAGATATTCATTAAGATGCATAGAGCAGAGTATGCTGAGAAGCAG
CTTAGAGTGATGCAACAGATTGATGAAGCCAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGNTTATTA
   
Sequence Name 09-K109633-0022-175-D03C-8409
GenBank Accession LN490223 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K109633-0022-175-D03C-8409
ATTGAATATATCCTGGCTTCATCAATCTGTTGCATCACTCTAAGCTGCTTCTCAGCATAC
TCTGCTCTATGCATCTTAATGAATATCTGAACATTCAAGGCATACCTAGAAAAACAATAG
CAATGAGCCATCTAAGAACATGTTTTAAAACACAGTATAAATACTCAATCCACCAAATAA
AAATCGAAAAAATCTTACAAATCCATAGTTCCTCCAGCATGAGTATGCTTCAAAGTTTCA
TTATAATCTTCTTCATGCATGAAAATAATACCAGCAATCAATCGTAATGTATCATTGTTT
CCTATTGTTGAATCTGCTAACCATTCCTTCAAGCTTGAAATTGTTGATTCCTGAACCAAT
AACAATCGTTCCATATAAGCATTTGAATCTCTTTACAACATGTTTCGTGAACACGAATCA
AAGCCGTAATTACCTTATTTTGAGGAGTCGATAGATACATAGCGA
Primers used for PCR 14df - 8474, show primer details
   
Sequence Name 22-K109674-0022-175-D03C-14df
GenBank Accession LN496153 [GenBank]
Sequencing primer gene specific primer, 14df: 5'-GCTACTCCACTACAAGCAGTCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K109674-0022-175-D03C-14df
CAAATAAGGTATTACGGCTTTGATTCGTGTTCCGAAACATGTTGTAAAGAGATTCAAATG
CTTATATGGAACGATTGTTATTGGTTCAGGAATCAACAATTTCAAGCTTGAAGGAATGGT
TAGCAGATTCAACAATAGGAAACAATGATACATTACGATTGATTGCTGGTATTATTTTCA
TGCATGAAGAAGATTATAATGAAACTTTGAAGCATACTCATGCTGGAGGAACTATGGATT
TGTAAGATTTTTTCGATTTTTATTTGGTGGATTGAGTATTTATACTGTGTTTTAAAACAT
GTTCTTAGATGGCTCATTGCTATTGTTTTTCTAGGTATGCCTTGAATGTTCAGATATTCA
TTAAGATGCATAGAGCAGAGTATGCTGAGAAGCAGCTTAGAGTGATGCAACAGATTGATG
AAGCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCA
   
Sequence Name 06-K109673-0022-175-D03C-8409
GenBank Accession LN488856 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K109673-0022-175-D03C-8409
TCGGACATGAGCCATTACAATTGAATATATCCTGGCTTCATCAATCTGTTGCATCACTCT
AAGCTGCTTCTCAGCATACTCTGCTCTATGCATCTTAATGAATATCTGAACATTCAAGGC
ATACCTAGAAAAACAATAGCAATGAGCCATCTAAGAACATGTTTTAAAACACAGTATAAA
TACTCAATCCACCAAATAAAAATCGAAAAAATCTTACAAATCCATAGTTCCTCCAGCATG
AGTATGCTTCAAAGTTTCATTATAATCTTCTTCATGCATGAAAATAATACCAGCAATCAA
TCGTAATGTATCATTGTTTCCTATTGTTGAATCTGCTAACCATTCCTTCAAGCTTGAAAT
TGTTGATTCCTGAACCAATAACAATCGTTCCATATAAGCATTTGAATCTCTTTACAACAT
GTTTCGTGAACACGAATCAAAGCCGTAATTACCTTATTTTGAGGAGTCGATAGATACATA
GCGAGGAGTTTGACTGCTTGT

Last Updated on 06.12.2018 16:11