Sequences from confirmation amplicon


Confirmation sequences for line 175D04 and hit At1g12860 are shown.
Line ID 175D04
Locus At1g12860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4386024
Primers used for PCR 09sa - 8474, show primer details
   
Sequence Name 45-K116229-0022-175-D04C-09sa
GenBank Accession LN505649 [GenBank]
Sequencing primer gene specific primer, 09sa: 5'-CACTGCTTTGATTTGTTCAGGTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K116229-0022-175-D04C-09sa
GCGGAAAAACATTCCAAAGCAAAAACCATTCGAAAACAGCTAATTCACCGCCTTGGTTGA
ACATCCAATCCTAAGTTATCCAAAGCTTCTCATGGTGGAAAGTAAAAGACCTGGTCTACG
TCCACAGAAACATGTGTATGTTTCACTGCCTTTTCCTTTCTCTTAATCTAACCTCAACCT
ATATCAAAACAAAGAAGCGTAAATCGCTTTCCAGGATATATTCAATTCGTAAATGGCTTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTTCATTCAA
   
Sequence Name 05-K116228-0022-175-D04C-8409
GenBank Accession LN488500 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K116228-0022-175-D04C-8409
GGTTAGATTAAGAGAAGGAAAGGCAGTGAACATACACATGTTCTGTGGACGTAGACCAGG
TCTTTTACTTTCCACCATGAGAGCTTTGGATAACTTAGGATTGGATGTTCAACAAGCGGT
GATTAGCTGTTTCAATGGTTTTGCTTTGGATGTTTTCCGCGCTGAGGTAATATAGTTGTT
AAGATGCTATGCTGATCACTGATTAGTAAAACTAGCAGTCAAAGAATAGTTATTTGAGAG
AATGATTAGTATACAAGTAACAACATGTTTTGTGTTTCTAATCAGCAATGTCAAGAAGAC
CATGACGTGTTACCTGAA

Last Updated on 06.12.2018 16:11