Genotyping and primer details


Genotyping and primer details for 175D04 - At1g12860
Line ID 175D04
Locus At1g12860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4386024 of line 175D04 Chr1:4386024
   
Insertion allele amplicon 8474 - 09sa
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 09sa: 5'-CACTGCTTTGATTTGTTCAGGTAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4386356
   
wt amplicon (over InsPos) 07sq - 09sa
wt amplicon start Chr1:4385431
wt amplicon end Chr1:4386356
wt amplicon size 926 bp
Locus specific primer 09sa: 5'-CACTGCTTTGATTTGTTCAGGTAA-3'
2nd locus specific primer 07sq: 5'-AATCGAGCTACGAGAGAGAGATTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11