Sequences from confirmation amplicon


Confirmation sequences for line 175D10 and hit At3g20760 are shown.
Line ID 175D10
Locus At3g20760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7257725
Primers used for PCR 30ra - 8474, show primer details
   
Sequence Name 29-K116053-0022-175-D10C-30ra
GenBank Accession LN499434 [GenBank]
Sequencing primer gene specific primer, 30ra: 5'-AACCTTGTTTCCTCGACAGTTCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K116053-0022-175-D10C-30ra
CATCAGCTCCTCTCCCACTGCCACCATTTTGCTCATTGGCTATGCAATAATCACCAAAAC
CCCAAAATCATTAGCTAACATATACCATCTAGACACTTACTAAAATAAAATGAAACACTA
ATCTATACCTCCCAATCTTTGTAATCAAATCTAAGAACAAAATGGTTATAAGCGACCTCC
CCTGACAGCACCAGGTTTGCTGCAGGAGCATTCCTTGGCTCTGAAGACAAAGAAACCAGT
TTTTTGGGGTTAGAGGGTGAAATCAACTACCAAAATCAGACACAAGGAAGGAAAGGTAAA
TCTTACCAACAAAATGAGAACCATTGTTATCAACAGTTATCTCAACACGTCCATCTTTCA
CCAGGAATGAAAGTGCGAACATATTCTCTGCTGTCTGAATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 12-K116052-0022-175-D10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K116052-0022-175-D10C-8409
GTCGCTTGGGGTCAGAGGGTGAAATCATCTACTTACATCAAACGCGAGGAAGGAATGGTT
GATCCAACCAACTTTATGAAAACCCTTGTTATCAACAGTTATCTCAACGCGTCCGGCGTT
CTGCAGGAATGAGCGTGCCAACTTATCCACTGCTGTCCGAAGATAATCGAATGTAAAGGG
CTTCATGTCCGGGAAATCTACGCGGATCACCTATGAGTATGATGGTCAATATGGATAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATCTCCGCCCGG

Last Updated on 06.12.2018 16:11