Sequences from confirmation amplicon


Confirmation sequences for line 175F11 and hit At3g52970 are shown.
Line ID 175F11
Locus At3g52970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19642446
Primers used for PCR MS55 - 8474, show primer details
   
Sequence Name 23-K017176-022-175-F11C-MS55
GenBank Accession LN496349 [GenBank]
Sequencing primer gene specific primer, MS55: 5'-GTCAGTGTTCTTGGGTTGTGTAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K017176-022-175-F11C-MS55
CGCTAGTGCCCACTCCAACGTGCTCGTTGTCGTATCCGTTCCAGCCGTAAACATTTCCTA
TCAATAAACTTTATTAATTTGTACATATGATTGTAAGAATGTTGGTAATAATTAATTAAG
GTATGCATAACTTACGAAAACAATAACGTTGATATCTCTTGATGAGAAGCTAGAAGGTTC
TTCGTCGACTCCATCGCCACCACGAAACTCCAAAAGAACATCTAAGTAATCTTTCGTCTT
CTCGTCGCTCTTTTCTCTCTCCCTAACCTCCGTTCGTTCTCTGATGAACTCTCCGGCGAT
CTCAAACGCTTTTTCCACGTGGAATTGCGTTTTTCTCCTGATTCCTTGAGGGTCAAGAAA
CCTTAGCAACGGGAAGAAATCGGCAACGTTTGGTTTTCCGGCGAACTCCATCACTTTCCC
GGTGTGGTAAAAGAATTCGGATCCTCTTTTTGAATCAGGATCAAGCAAATCTCTCGACAC
ATCAAGAACATGGATCAGCAATGAGTATGATCTACATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGA
CAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAA
ATCTTTATTTCGACGTGTCTACAT
   
Sequence Name 27-K017176-022-175-F11C-8409
GenBank Accession LN498162 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K017176-022-175-F11C-8409
TGTAGATTTCCCGGACATGTAGATCATACTCATTGCTGATCCATGTTCTTGATGTGTCGA
GAGATTTGCTTGATCCTGATTCAAAAAGAGGATCCGAATTCTTTTACCACACCGGGAAAG
TGATGGAGTTCGCCGGAAAACCAAACGTTGCCGATTTCTTCCCGTTGCTAAGGTTTCTTG
ACCCTCAAGGAATCAGGAGAAAAACGCAATTCCACGTGGAAAAAGCGTTTGAGATCGCCG
GAGAGTTCATCAGAGAACGAACGGAGGTTAGGGAGAGAGAAAAGAGCGACGAGAAGACGA
AAGATTACTTAGATGTTCTTTTGGAGTTTCGTGGTGGCGATGGAGTCGACGAAGAACCTT
CTAGCTTCTCATCAAGAGATATCAACGTTATTGTTTTCGTAAGTTATGCATACCTTAATT
AATTATTACCAACATTCTTACAATCATATGTACAAATTAATAAAGTTTATTGATAGGAAA
TGTTTACGGCTGGAACGGATACGACAACGAGCACGTTGGAGTGGGCACTAGCGGAGCTTC
TACACAACCCAA

Last Updated on 06.12.2018 16:11