Sequences from confirmation amplicon


Confirmation sequences for line 176C11 and hit At4g21040 are shown.
Line ID 176C11
Locus At4g21040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11235334
Primers used for PCR MZ05 - 8474, show primer details
   
Sequence Name 57-K015910-022-176-C11C-8409
GenBank Accession LN509258 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K015910-022-176-C11C-8409
GATTTCCCGGACATGAAGCCATTTACAATTGAATTTCGTAATTTACAATTTTGTTACTGG
ACTCATGGTGGGGCTTTAAGAAACGTACCAATTGGTGGGAGTAGTCGTGCCAAGCGGACA
AGGATAAATCAACCTTCAGTTGCTCAGATGGTTTCTGTTGGAATCCAACCAGGTAGCCAT
AAACCTTTCTTTAATGTTCAAGAAAATAATGACTTTGTTGGATCTTTTGGTGCTTCATCT
TCATCTTTTGTTGCTGCTGTAGGGAACCGTTTTAGTTCTTTGTCTCATATTCATGGTGGT
ATGGTAACAAATGTGCATCCAACTCAAACTTTTCGACCAAATCATCGCCTAGCTTTCCAT
AATGGATCATTTGAGCAAGATTATTATGATGTTGGGTCTGATAATCTTTTGGTAAACCAA
CAAGTTGGTGGATATGTTGATAATCACAACGGTTATCACATGAATCAAGTGGATCA
   
Sequence Name 58-K015910-022-176-C11C-MZ05
GenBank Accession LN509564 [GenBank]
Sequencing primer gene specific primer, MZ05: 5'-GGTTCCAGTTGTATTGATCCACTT-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K015910-022-176-C11C-mz05
ATGTGATAACCGTTGTGATTATCAACATATCCACCAACTTGTTGGTTTACCAAAAGATTA
TCAGACCCAACATCATAATAATCTTGCTCAAATGATCCATTATGGAAAGCTAGGCGATGA
TTTGGTCGAAAAGTTTGAGTTGGATGCACATTTGTTACCATACCACCATGAATATGAGAC
AAAGAACTAAAACGGTTCCCTACAGCAGCAACAAAAGATGAAGATGAAGCACCAAAAGAT
CCAACAAAGTCATTATTTTCTTGAACATTAAAGAAAGGTTTATGGCTACCTGGTTGGATT
CCAACAGAAACCATCTGAGCAACTGAAGGTTGATTTATCCTTGTCCGCTTGGCACGACTA
CTCCCACCAATTGGTACGTTTCTTAAAGCCCCACCATGAGTCCAGTAACAAAATTGTAAA
TTACGAAATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAAC

Last Updated on 06.12.2018 16:11