Sequences from confirmation amplicon


Confirmation sequences for line 177A09 and hit At1g24470 are shown.
Line ID 177A09
Locus At1g24470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8675783
Primers used for PCR JK56 - 8474, show primer details
   
Sequence Name 34-K100939-0022-177-A09C-8409
GenBank Accession LN501340 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K100939-0022-177-A09C-8409
TAAAATGACAACTAACATTTTTACTTTGAAAGCAGTAAATAGTAGTTTTCCTTTTCATTG
CATTAAATATTACTACTGTCTAGATATTTGCATTAATCATGATTTAAATTGTTTAATTTG
CTCAATTATTCATCTAACTATATCATTATATTACCTACCTGAGCGCAGATTGTCTCTGTC
ACATTAAGAAATCTCTAAACACGTGTATGCAGTTATGTGGATGCACTATCAAGATCTCTA
CATGTG
   
Sequence Name 34-K100946-0022-177-A09C-JK56
GenBank Accession LN501341 [GenBank]
Sequencing primer gene specific primer, JK56: 5'-ATCAACAAGGTTATCCGGTACAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K100946-0022-177-A09C-JK56
TGAGCCCAAAACGGCGAGCAACGTGATCCAATTCCGATCTGCGCCACCGCCGCTTTTGCG
TAGACTTCAGGCGACGGTACAAATAAGCTAGGTTTGTCTATAGCTGCCACTTCTGACACC
ATCCTTGTTGACACGTATAAGGGAACCTGTCCATTTGATTTACACACGTACGTGCATTAT
AACATTAAATCTNTTACGTATTACCTGAGGGAGTACAAAATATGCTATAATACATATATT
GGATAGTCTTTTGAATACAAAAAAAGAAGAAAAAGTTCCTGGCACTGGAACTTCATACCA
AACTGGTTATATTCCACATGTAGAGATTTTGATTGTGGATCCACCTTACTGCCTACCCCT
GGTTAGAGATTTCTTAATGTGACAGAAACCATCTGCGCTC

Last Updated on 06.12.2018 16:11