Sequences from confirmation amplicon


Confirmation sequences for line 177B09 and hit At3g51150 are shown.
Line ID 177B09
Locus At3g51150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19003530
Primers used for PCR 04ie - 8474, show primer details
   
Sequence Name 48-K112758-0022-177-B09C-04ie
GenBank Accession LN506544 [GenBank]
Sequencing primer gene specific primer, 04ie: 5'-ATTACCTTTTCGATTTGGAGGTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K112758-0022-177-B09C-04ie
ACAGAGCTTGAGAGGGCTGCTCACTCGCTCTCCAGTTTAGCCAATTCTCTCTGTAAATGT
CTAACCAGAGCTTTATCAGACATGACGACGTTGACTTGTGCATTTGTTGTCACCTCCTTT
GCACAGCTTGCGAATAACAATGTGTTTCTTGATTGCTCAACGTGTACTCTTGCAGGACTC
AGTGTGCAGATTATGGAAGTTCTGGCGTTTCCTCCCAAGGAAGTCTGAAGTATGCGTGTC
AGCTTTGAATCTCTGAATGGAATGTGCCCATTTTTTCCTTTACTGCATTAAGCTAGCCAC
ATAATTCAGTAATGTAAAAAGAAGCAAACATTAGATGTTAATAACAGCAATCTGCTAATA
ACTTGGTAAACTGGCGTTACTGGGCGAAAGTCCTATGTTTCAGCTTGGAACTCAACTCTC
AAAGACAATGATTAGCTGCTTAACCATCATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 08-K112757-0022-177-B09C-8409
GenBank Accession LN489802 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K112757-0022-177-B09C-8409
GATATGATGGTTAAGCAGCTAATCATTGTCTTTGAGAGTTGAGTTCCAAGCTGAAACATA
GGACTTTCGCCCAGTAACGCCAGTTTACCAAGTTATTAGCAGATTGCTGTTATTAACATC
TAATGTTTGCTTCTTTTTACATTACTGAATTATGTGGCTAGCTTAATGCAGTAAAGGAAA
AAATGGGCACATTCCATTCAGAGATTCAAAGCTGACACGCATACTTCAGACTTCCTTGGG
AGGAAACGCCAGAACTTCCATAATCTGCACACTGAGTCCTGCAAGAGTACACGTTGAGCA
ATCAAGAAACACATTGTTATTCGCAAGCTGTGCAAAGGAGGTGACAACAAATGCACAAGT
CAACGTCGTCATGTCTGATAAAGCTCTGGTTAGACATTTACAGAGAGAATTGGCTAAACT
GGAGAGCGAGTTGAGCAGCCCTCGTCAAGCTCTTGTTGTGTCTGACACAACTGCATTACT
GAAGGAGAAAGACCTCCAAATCGAANAGGTAA

Last Updated on 06.12.2018 16:11