Sequences from confirmation amplicon


Confirmation sequences for line 177D02 and hit At4g34870 are shown.
Line ID 177D02
Locus At4g34870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16614381
Primers used for PCR JK29 - 8474, show primer details
   
Sequence Name 21-K025043-022-177-D02C-8409
GenBank Accession LN495506 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K025043-022-177-D02C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATTTCCCGCCTTCGGTTTAAACTAT
TTGGAGAGGTTCTTATAGAGAGGATTTAATTTCTAAAACGGTGAATCTGAAAATGGAAAT
TATGTTGGTGACCGTTGATTTATGACACGTGTAGAGCTGTGATGATATGGAAATTAACCG
TGGTTGAATCGGATCTGACTAGGGTTGAAGTCGCGTGGATCCTAGGCTCCTTTCCATAAT
TGATTTGATTCTTTTTAGATGGACCGACAGAATTACGGCGTTGGAGTTTAGGTGGGCCGA
CTCCAAAAGCCTAAAGCCCAATATCTTATTTTAACATTACAATTTTTTGGTGTATGATTG
AGCATTTTTTTGCCACAACACTCGTTATCCGATTCTATTGACAC
   
Sequence Name 22-K025043-022-177-D02C-JK29
GenBank Accession LN495953 [GenBank]
Sequencing primer gene specific primer, JK29: 5'-GGGAGTGTCAATAGAATCGGATAA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K025043-022-177-D02C-JK29
AGTGTTGTGGCAAAAAAATGCTCAATCATACACCAAAAAATTGTAATGTTAAAATAAGAT
ATTGGGCTTTAGGCTTTTGGAGTCGGCCCACCTAAACTCCAACGCCGTAATTCTGTCGGT
CCATCTAAAAAGAATCAAATCAATTATGGAAAGGAGCCTAGGATCCACGCGACTTCAACC
CTAGTCAGATCCGATTCAACCACGGTTAATTTCCATATCATCACAGCTCTACACGTGTCA
TAAATCAACGGTCACCAACATAATTTCCATTTTCAGATTCACCGTTTTAGAAATTAAATC
CTCTCTATAAGAACCTCTCCAAATAGTTTAAACCGAAGGCGGGAAATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTA
TTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGC
GAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11