Sequences from confirmation amplicon


Confirmation sequences for line 177D11 and hit At4g03070 are shown.
Line ID 177D11
Locus At4g03070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1359460
Primers used for PCR IW16 - 8474, show primer details
   
Sequence Name 52-K016445-022-177-D11C-8409
GenBank Accession LN507662 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K016445-022-177-D11C-8409
TAGATTTCCCGGACATGAAGCCATTTCCAAAAACAGGAGTTATCATTGATTCACCAGAAG
AACTTGTCGACAAAGAACATCCACGAATATTCAAACCCTTTGAATACACAGATTTTCTTC
ACTTCTTTCAGACAGAAGCTGGACGTATAGCTCAATCAGCTCTCCATGCTTTCGCTGCCT
TTTGAATCAGAAGTATGTATGAATGTATAACTTCATGTTGTTGTGTTGTAGTTTTAACAT
TCCAAAAACCAAATCTATGTTTGAAGAAATATAAATCATCTCTCACTAAAGAGTTTTTTA
GAAGAAAAGCAAAAAAATAGTACTATACCATTCTCACTCTCTGGTGGTATAGTGGATTAT
ATTACACATGTGCGGCAGAGTATATGAACACTGCAAATTTTGAAACATAAAATCTTACTA
TACTTGTATAAAGTTGTACCAAAATCCAATATTGGTTGACTTCATGAGTTGCAGGCAGAA
ACCGGGAATGCAATCAGATTCAGATGTATGCAAGCGAGCTTCTGTCAAAATCTGGTTGTG
GCCTGCCTCGTGTTGGAGTCGGCGGTCTCTGGATATTCAGCTCCTGCAAAAGATAAATTG
TGTACTTTCAGTGTCTCTGGTTCGTAGCCAAAAAAAGATATAGCTACATAAACTCTCCTA
GGACTATCCATATCACATAACTACCAACTAAGATCAAGTTGCTCAGTTTGTGGCTGAAAC
AAAGACATTATGTGCTTCAC
   
Sequence Name 56-K016445-022-177-D11C-IW16
GenBank Accession LN508960 [GenBank]
Sequencing primer gene specific primer, IW16: 5'-CCTAGCATATACCAGTGAAGCACA-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K016445-022-177-D11C-iw16
TAATGTCTTTGTTTCAGCCACAAACTGAGCAACTTGATCTTAGTTGGTAGTTATGTGATA
TGGATAGTCCTAGGAGAGTTTATGTAGCTATATCTTTTTTTGGCTACGAACCAGAGACAC
TGAAAGTACACAATTTATCTTTTGCAGGAGCTGAATATCCAGAGACCGCCGACTCCAACA
CGAGGCAGGCCACAACCAGATTTTGACAGAAGCTCGCTTGCATACATCTGAATCTGATTG
CATTCCCGGTTTCTGCCTGCAACTCATGAAGTCAACCAATATTGGATTTTGGTACAACTT
TATACAAGTATAGTAAGATTTTATGTTTCAAAATTTGCAGTGTTCATATACTCTGCCGCA
CATGTGTAATATAATCCACTATACCACCAGAGAGTGAGAATGGTATAGTACTATTTTTTT
GCTTTTCTTCTAAAAAACTCTTTAGTGAGAGATGATTTATATTTCTTCAAACATAGATTT
GGTTTTTGGAATGTTAAAACTACAACACAACAACATGAAGTTATACATTCATACATACTT
CTGATTCAAAAGGCAGCGAAAGCATGGAGAGCTGATTGAGCTATACGTCCAGCTTCTGTC
TGAAAGAAGTGAAGAAAATCTGTGTATGCAGAGGGTTTGAATATGCGTGGATGTTCTTTG
ACGACAAGTTCTTCTGGTGAATCAATGATAACTCCTGTTTTTGGAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCGATATGGAGAAAAA

Last Updated on 06.12.2018 16:11