Sequences from confirmation amplicon


Confirmation sequences for line 177E02 and hit At5g25160 are shown.
Line ID 177E02
Locus At5g25160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8687551
Primers used for PCR KT40 - 8474, show primer details
   
Sequence Name 73-K100816-0022-177-E02C-8409
GenBank Accession LN512849 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K100816-0022-177-E02C-8409
GAGCCATTTACATTGTTTTTGATATCTCAATCTTGATATGGATGAGGAAACTATTGAAGC
GTCCATGAGAGCTAAAAAGGGATAGATTTTTTCAGGAGATGATTTTTTTCTTTCTCTTTC
TCTGTCTCTTTCTATCAATAATAGCTATGAGAAGAGGTTTATATCAGTGAATGGGAGCAG
AAGTAGATGGTATTAGAAAGGAATAGACAAAGGAAACAAAGACCCTACACTAGAACATTT
TCTTAGGGTTTTGTAATAAGTGGACCAAAGTACTATGATGAGAGCGATAGGTAGGTTTGG
TTTGCTGTCACACCCTTCTCACAAAACACTTTAAAGCTAGTTGATTGGAACTCCACCATC
TAAATACCTCTATCTATATATATGACTCTNACCCTTTTTGGCTGCTTCCTCCTTCAATAG
CCCAACAAA
   
Sequence Name 73-K100828-0022-177-E02C-KT40
GenBank Accession LN512851 [GenBank]
Sequencing primer gene specific primer, KT40: 5'-ATTTGTTGGACTATTGAAGGAGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K100828-0022-177-E02C-KT40
ATAGAGGTATTTGATGGTGGAGTTCCATCAACTAGCTTTAAAGTGTTTTGTGAGAAGGGT
GTGACAGCAAACCAAACCTACCTATCGCTCTCATCATAGTACTTTGGTCCACTTATTACA
AAACCCTAAGAAAATGTTCTAGTGTAGGGTCTTTGTTTCCTTTGTCTATTCCTTTCTAAT
ACCATCTACTTCTGCTCCCATTCACTGATATAAACCTCTTCTCATAGCTTTTATTGATAG
AAAGAGACAGAGAAAGAGAAAGAAAAAAATCATCTCCTGAAAAAATCTATCCCTTTTTAG
CTCTCATGGACGCTTCAATAGTTTCCTCATCCATATCANGATTGAGATATCAAAAACAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11