Sequences from confirmation amplicon


Confirmation sequences for line 177E04 and hit At4g11990 are shown.
Line ID 177E04
Locus At4g11990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7189311
Primers used for PCR AP09 - 8474, show primer details
   
Sequence Name 16-K106147-0022-177-E04C-AP09
GenBank Accession LN493438 [GenBank]
Sequencing primer gene specific primer, AP09: 5'-ACCACATGTGTGTTCTCTTCAGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K106147-0022-177-E04C-AP09
GTAGGATCGTTTGGAATTTGCTCAAGTACCACCTTGAATGAATCTAAACACCCCAGACTT
CATTCCATTTCCTGAAACAAAACAAGATTCCATTTTACATCAACCATAACTAGTAAACTC
TTCAAGTTTGTTATCCAAAACCTAAACAATGCTAGTTTAGTGATACAGAAACATAATCCA
AAACATCCTAAATTCACCTGTTTCATTCACATCTGGTTGCTGAGGGATTTCTCTGTCACT
CTCCTGAACTTCTACTGTGACTTCCCCATCTTCTGATTTTGACAAAGACTCGGTCTTTTC
ATCATAAACCTCTTCTATCATTAACAGTTTCATTGCAAAAGCTGACAAAACACAATAAAT
TAGACATAAACACTCTTTAGTTCTAACAAGACATTAGTCCTCTGCATTTGTAATGAAATT
CTCAATTCAGTGAAACAAAATCAAATCTAATCATCAAAATAACAGATTCATAAAACACAA
ATGACGATTCAGGATCAGCAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCACGNTTATT
   
Sequence Name 32-K106148-0022-177-E04C-8409
GenBank Accession LN500591 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K106148-0022-177-E04C-8409
GCCATTTGCTGATCCTGAATCGTCATTTGTGTTTTATGAATCTGTTATTTTGATGATTAG
ATTTGATTTTGTTTCACTGAATTGAGAATTTCATTACAAATGCAGAGGACTAATGTCTTG
TTAGAACTAAAGAGTGTTTATGTCTAATTTATTGTGTTTTGTCAGCTTTTGCAATGAAAC
TGTTAATGATAGAAGAGGTTTATGATGAAAAGACCGAGTCTTTGTCAAAATCAGAAGATG
GGGAAGTCACAGTAGAAGTTCAGGAGAGTGACAGAGAAATCCCTCAGCAACCAGATGTGA
ATGAAACAGGTGAATTTAGGATGTTTTGGATTATGTTTCTGTATCACTAAACTAGCATTG
TTTAGGTTTTGGATAACAAACTTGAAGAGTTTACTAGTTATGGTTGATGTAAAATGGAAT
CTTGTTTTGTTTCAGGAAATGGAATGAAGTCTGGGGTGTTTAGATTCATTCAAGGTGGTA
CTTTGAGCAAAGTTCCAAACGATTCCTTACATAAAGGTGAACACTTTTGCTAACATGACT
GAAGAGAACACCCATGTGG

Last Updated on 06.12.2018 16:11