Sequences from confirmation amplicon


Confirmation sequences for line 178B09 and hit At1g35540 are shown.
Line ID 178B09
Locus At1g35540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:13110388
Primers used for PCR 42st - 8474, show primer details
   
Sequence Name 45-K116569-0022-178-B09C-42st
GenBank Accession LN505656 [GenBank]
Sequencing primer gene specific primer, 42st: 5'-TAAATTTTGGTTTAGGATATGTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K116569-0022-178-B09C-42st
ATCTTCATGGTAATCATGGAGATTCAGACACATCTATAGAGGTAATAATTCCTTTTATTA
TATAATTAAAACACAGCTATTGAGGTAATAAATCCTAGAGAAGTAACTAATAATCTTCTT
ATTCCATGTAGGTACAGCGCAAAGACATTTGTTAACAATTGGTTGGAATGCATTCACAAC
ATCAAAAAAATTGGTTGAAGGAGATGTTATTGTATTCGTTAGGTATAATATTGGTTCTTC
TAATATATCTTTGTGTTCTTGAGTTTTGGACTTTAAACTATATACTTGTGGTTTTAGGGG
AGAGACTGGAGAGTTACGAGTTGGTATCAGACGAGCAGGACATCAACAAGGGAATATACC
TTCATCAATAGTATCAATAGAAAGTATGAGACATGGGATAATTGCTTCTGCAAAGCATGC
TTTTGATAACCAATGTATGTTCATTGTGGTTTACAAGCCAAGGTAATTTCAAAGAACCAT
TTGAACTCACATTTTAGATTATATTGATTTATCAATAAATTAGTTTCTTCAACATGTAGG
TTTATATTTTGTGTCTTTATAAGTATCAGGTCAAGTCAATTTATTGTCAGTTACGACAAA
TTCTTAGACGTTGTGAACAATAAGTTCAATGTCGGTTCAAGATTTACAATGCGGTTTGAG
GGTGATGATTTCTCTGAAAGAAGGTAACATTAGATGAAAAAAAAAATTGCAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 16-K116568-0022-178-B09C-8409
GenBank Accession LN493589 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K116568-0022-178-B09C-8409
CATTTGCATTTTTTTTTTCATCTAATGTTACCTTCTTTCAGAGAAATCATCACCCTCAAA
CCGCATTGTAAATCTTGAACCGACATTGAACTTATTGTTCACAACGTCTAAGAATTTGTC
GTAACTGACAATAAATTGACTTGACCTGATACTTATAAAGACACAAAATATAAACCTACA
TGTTGAAGAAACTAATTTATTGATAAATCAATATAATCTAAAATGTGAGTTCAAATGGTT
CTTTGAAATTACCTTGGCTTGTAAACCACAATGAACATACATTGGTTATCAAAAGCATGC
TTTGCAGAAGCAATTATCCCATGTCTCATACTTTCTATTGATACTATTGATGAAGGTATA
TTCCCTTGTTGATGTCCTGCTCGTCTGATACCAACTCGTAACTCTCCAGTCTCTCCCCTA
AAACCACAAGTATATAGTTTAAAGTCCAAAACTCAAGAACACAAAGATATATTAGAAGAA
CCAATATTATACCTAACGAATACAATAACATCTCCTTCAACCAATTTTTTTGATGTTGTG
AATGCATTCCAACCAATTGTTAACAAATGTCTTTGCGCTGTACCTACATGGAATAAGAAG
ATTATTAGTTACTTCTCTAGGATTTATTACCTCAATAGCTGTGTTTTAATTATATAATAA
AAGGAATTATTACCTCTATAGATGTGTCTGAATCTCCATTGATTACCATGAAGATCTATA
GCAAGAATTTCTTGTGTCGGTAGAGGCTGTGACATATCCT

Last Updated on 06.12.2018 16:11