Sequences from confirmation amplicon


Confirmation sequences for line 179A06 and hit At1g71360 are shown.
Line ID 179A06
Locus At1g71360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26892954
Primers used for PCR 05wm - 8474, show primer details
   
Sequence Name 30-K118761-0022-179-A06C-05wm
GenBank Accession LN499928 [GenBank]
Sequencing primer gene specific primer, 05wm: 5'-TTCTCTTTCTCATTCTCAGCCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K118761-0022-179-A06C-05wm
TCCGCCACTTTCTATCTCCATCGCCTCTTTCTTCGTATTCTCTTCCCTCTCCTTCATTCC
TTCTACCTCTAGTCTCATAGTCTCCACCTCCTTCTCTCTCTTGCTCGCCTCCAGGTCCAT
CTCCTTGAATATCATCCCGTATTTCAAGCTCCGTTCCTCCAAGTAACTCTCAAGAACTGA
TAAGCTCACGTCCAGCGACCTTATCTTCTGCATCAGTATCTTCAAGACAGTGTCCCCCGG
CATTCTGCTTCCTGGTTGATGTTTTATCTCTTCCACCGGATCTGGCAGCTTTCTTTTCTC
AAGTGATACTTCATCTTTCACAACCGCATTCTCTGGTGAGGCTTCTTGTTCCTTCTCCTT
CTGTTTACTCTTGTCTTCATCGCTTTCGAAGGACTCTTTTGCTTGCATTGTCTTCTTCTC
TTTCTGCTCAGTGTCTCCTTCTTGGAGTTTCAATATGTTCTTGTCTTGGATAGATATCAA
TAGTGATAATAGTGATTTCTCCATATTGAATTTCCAGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 05-K118760-0022-179-A06C-8409
GenBank Accession LN488567 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K118760-0022-179-A06C-8409
TTTACATTGAATATATCCTGGAAATTCAATATGGAGAAATCACTATTATCACTATTGATA
TCTATCCAAGACAAGAACATATTGAAACTCCAAGAAGGAGACACTGAGCAGAAAGAGAAG
AAGACAATGCAAGCAAAAGAGTCCTTCGAAAGCGATGAAGACAAGAGTAAACAGAAGGAG
AAGGAACAAGAAGCCTCACCAGAGAATGCGGTTGTGAAAGATGAAGTATCACTTGAGAAA
AGAAAGCTGCCAGATCCGGTGGAAGAGATAAAACATCAACCAGGAAGCAGAATGCCGGGG
GACACTGTCTTGAAGATACTGATGCAGAAGATAAGGTCGCTGGACGTGAGCTTATCAGTT
CTTGAGAGTTACTTGGAGGAACGGAGCTTGAAATACGGGATGATATTCAAGGAGATGGAC
CTGGAGGCGAGCAAGAGAGAGAAGGAGGTGGAGACTATGAGACTAGAGGTAGAAGGAATG
AAGGAGAGGGAAGAGAATACGAAGAAAGAGGCGATGGAGATGAGAAAGTGGCGGATGAGA
GTGGAGACAGAGCTTGAGAAGGCTGAGAATGAGAAAAGAGA

Last Updated on 06.12.2018 16:11