Sequences from confirmation amplicon


Confirmation sequences for line 179B12 and hit At1g52580 are shown.
Line ID 179B12
Locus At1g52580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19588536
Primers used for PCR RK45 - 8474, show primer details
   
Sequence Name 43-K015903-022-179-B12C-8409
GenBank Accession LN504659 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K015903-022-179-B12C-8409
GATTTCCCGCGATTCTTACCACGTGTGGACAATTCTGCTCATTTTGGAGGATTCCTAGCT
GGTTTCTTCCTCGGGTTCGTCCTTCTTCTCCGTCCGCAGTACGGATACGTGAACCCAAAA
TACATTCCTCCTGGTTATGACATGAAACACAAGAAATCAAAACACAAGTGTTATCAACAC
ATCTTCAGATTTACATCTCTAGCTATCCTTCTTGCGGGGTAAGTTAACGAAAGAGATTTA
ATGAACCATTGTTCAAAAGTAGCCGAATTCTCCATCAACACATAAACAAAACCCTCAGTG
TTGTGAAAAGTTTCATCCCTTACCTGTAAATAATTGAAATTTTCCTTGAAATTTCTGCAG
ATTCATTGCGGGATACACTAAGCTTCTTAGAGAACATACCATACAGAGCATGCCATTCAG
AGATTTCAATTAGCAGCTTGTAGCCAGCAGACCAGTGAGACATTCTCTGAAAAAAGACAA
TTTCACACAATCCCAAGTTTTTTTCCATATATATAACACAAATATCAACGTCGGAAAGG
   
Sequence Name 44-K015903-022-179-B12C-RK45
GenBank Accession LN505019 [GenBank]
Sequencing primer gene specific primer, RK45: 5'-CAAATGTATAACCTTTCCGACGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K015903-022-179-B12C-rk45
ATGTTATATATATNGGAAAAAAACTTGGGATTGTGTGAAATTGTCTTTTTTCAGAGAATG
TCTCACTGGTCTGCTGGCTACAAGCTGCTAATTGAAATCTCTGAATGGCATGCTCTGTAT
GGTATGTTCTCTAAGAAGCTTAGTGTATCCCGCAATGAATCTGCAGAAATTTCAAGGAAA
ATTTCAATTATTTACAGGTAAGGGATGAAACTTTTCACAACACTGAGGGTTTTGTTTATG
TGTTGATGGAGAATTCGGCTACTTTTGAACAATGGTTCATTAAATCTCTTTCGTTAACTT
ACCCCGCAAGAAGGATAGCTAGAGATGTAAATCTGAAGATGTGTTGATAACACTTGTGTT
TTGATTTCTTGTGTTTCATGTCATAACCAGGAGGAATGTATTTTGGGTTCACGTATCCGT
ACTGCGGACGGAGAAGAAGGACGAACCCGAGGAAGAAACCAGCTAGGAATCCTCCAAAAT
GAGCAGAATTGTCCACACGTGGTAAGAATCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA

Last Updated on 06.12.2018 16:11