Sequences from confirmation amplicon


Confirmation sequences for line 179C08 and hit At3g15710 are shown.
Line ID 179C08
Locus At3g15710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5324362
Primers used for PCR 10ic - 8474, show primer details
   
Sequence Name 32-K112551-0022-179-C08C-10ic
GenBank Accession LN500647 [GenBank]
Sequencing primer gene specific primer, 10ic: 5'-CAATTGATTTTACTCTCATTTGCG-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K112551-0022-179-C08C-10ic
ATCTCACCAAATGTCACATCTTGAGAAGCTAATATGTATATAATGGGTTTGGTTTTGTTT
TTGGGTTTAGGTATGATCGTTACATCTGCATTGATAATTTGGAAGGCATTGATATGTGTC
ACTGGCAGTGAATCACCAGTGGTTGTTGTTCTCTCTGAAAGTATGGAACCTGGCTTTCAG
AGAGTAAGTCACATTATGTTTTACTCATTGCATTTGATTATGTGGTTGTTGGTTATTGAT
TTTGAGGCTAATGTGACACAGGGAGATATATTGTTCTTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 08-K112550-0022-179-C08C-8409
GenBank Accession LN489798 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K112550-0022-179-C08C-8409
TTACATTGAAGAACAATATATCTCCCTGTGTCCATTAGCCTCAAAATCAATAACCAACAA
CCACATAATCAAATGCAATGAGTAAAACATAATGTGACTTACTCTCTGAAAGCCAGGTTC
CATACTTTCAGAGAGAACAACAACCACTGGTGATTCACTGCCAGTGACACATATCAATGC
CTTCCAAATTATCAATGCAGATGTAACGATCATACCTAAACCCAAAAACAAAACCAAACC
CATTATATACATATTAAGCTTCTCAAGATGTGACATTTGGTGAGATTTAGATACCAATTT
CTCGAAATCTAAAACGCAAATGAGAGTAAAATCAATT

Last Updated on 06.12.2018 16:11