Sequences from confirmation amplicon


Confirmation sequences for line 179C08 and hit At5g67240 are shown.
Line ID 179C08
Locus At5g67240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26828181
Primers used for PCR 03ty - 8474, show primer details
   
Sequence Name 01-K118356-0022-179-C08C-03ty
GenBank Accession LN486779 [GenBank]
Sequencing primer gene specific primer, 03ty: 5'-CAATTAATGAGGAGGTCATCGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K118356-0022-179-C08C-03ty
ATTTTTAGGACATATGATGAGATCTCAGCCAAAGGGGTTTCAATACACTCTAAAATATCA
TACATTACAAATTTTACTTAATATTGAACCAACAAAATAAACATTTCAGAATTGAGAAAA
AATAGTAAAGACATAGATTTTGTTCTAGATGAATCAGAGATAAATACACTAGGAGTGGTC
TCAAAGACTTCTTCAATGTGTTAAACGGACCAATGAGGAGGCTTATATTAGCAATAAACC
TTCAAACCAAAAACATTAGTAAAATTAAAAATCTGAAATTTTATCAAACAAAGTAAGATT
AAATAGGAGGACAAAACAGTAATTAAAACATAAGCTTCCCGAACTAAAATTATTAAACAA
GAAATAAAAAAGAATAAAACCCTAAATTTCGATTGTCCAGGATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT
AT
   
Sequence Name 01-K118355-0022-179-C08C-8409
GenBank Accession LN486778 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K118355-0022-179-C08C-8409
ACAATCGAAATTTTAGGGTTTTATTCTTTTTTATTTCTTGTTAATAATTTTAGTTCGGGA
AGCTTATGTTTTAATTACTGTTTTGTCCTCCTATTTAATCTTACTTTGTTTGATAAAATT
TCAGATTTTTAATTTTACTAATGTTTTTGGTTTGAAGGTTTATTGCTAATATAAGCCTCC
TCATTGGTCCGTTTAACACATTGAAGAAGTCTTTGAGACCACTCCTAGTGTATTTATCTC
TGATTCATCTAGAACAAAATCTATGTCTTTACTATTTTTTCTCAATTCTGAAATGTTTAT
TTTGTTGGTTCAATATTAAGTAAAATTTGTAATGTATGATATTTTAGAGTGTATTGAAAC
CCCTTTTGGCTGAGATCTCATCATATGTTCCTAAAAATTTATGTGTTCAAGGTATCGATG
CATTCGATGACC

Last Updated on 06.12.2018 16:11