Sequences from confirmation amplicon


Confirmation sequences for line 179C09 and hit At3g09360 are shown.
Line ID 179C09
Locus At3g09360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2876609
Primers used for PCR 41fr - 8474, show primer details
   
Sequence Name 19-K111563-0022-179-C09C-41fr
GenBank Accession LN494817 [GenBank]
Sequencing primer gene specific primer, 41fr: 5'-TTAAAAGTTCGGCACAAGAAAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K111563-0022-179-C09C-41fr
GCAGAGTGACAGCTCCCATACAGGTGCTTTGGGTAGGGAGGAAAATATCTAAACTGCAAT
CCTCAAATAAAAGAGCACCAAAACTTACATATAGTTGTTCATCGTGGTTTAGGCTACTAA
TTCCTCCCTCGTTTTCTTCTCTAGCTGCTTTTTCCATTCGCTCTTTCTCTGCGCGCTGGA
AAGCAGGAGGATTGGACCCACCAACAAGTCCACCAGAAACATTTATGAACTGCATTAAAA
ATTGCAAAAGAAAACATTTGATTGTTAACTCTGGGAACCATTTGTAAATGATATATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
AGCATTATA
   
Sequence Name 03-K111556w-0022-179-C09C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K111556w-0022-179-C09C-8409
GCATTTCATTGAATATATCATTTAAAATGGTTCCCAGAGTTACAATCAAATGTTTTCTTT
TGCAATTTTTAATGCAGTTCATAAATGTTTCTGGTGGACTTGTTGGTGGGTCCAATCCTC
CTGCTTTCCAGCGCGCAGAGAAAGAGCGAATGGAAAAAGCAGCTAGAGAAGAAAACGAGG
GAGGAATTAGTAGCCTAAACCACGATGAACAACTATATGTAAGTTTTGGTGCTCTTTTAT
TTGAGGATTGCAGTTTAGATATTTTCCTCCCTACCCAAAGCACCTGTATTGGGAGCTTGT
TCACTTCTGCTTGTATGATATTATAATAAAATCTTTCTTGTGCCGAACTTTTA

Last Updated on 06.12.2018 16:11