Sequences from confirmation amplicon


Confirmation sequences for line 179E12 and hit At4g27980 are shown.
Line ID 179E12
Locus At4g27980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13921957
Primers used for PCR 02bk - 8474, show primer details
   
Sequence Name 19-K110037-0022-179-E12C-02bk
GenBank Accession LN494801 [GenBank]
Sequencing primer gene specific primer, 02bk: 5'-TTTTACTAGTTCCCGGAGAAGACA-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K110037-0022-179-E12C-02bk
TGGTGTCACCGAAGACCAACTCAAGAGAGGTTTATTTTCTCTTCCTACTTTTTGTTTCTA
TTATTTATTCTTTCTGTACATTCTCATTTTTGTATTTTTTCATGAAAAGTTTCTTCCTTT
ATGACCCATCTTTTGTTAACTAAAAAGTAGGGTTTATGTCTCTGAGAATATGGCAATGCA
AGGGGATTCAGTAGAATCAGACGTTACTGAGCAAAACAGTGAAGGGTATCTCAAAATCCT
AGACAAGATTCAGGCAAAATCTTCATCAATTTTTGAGTTTTCGCTTCAGTTTGATGACGT
GGTCGCCTCGCTGGAGAAGAAGGAAGAACGACTGAGAGTGGTTGAAATGAAGGAGAAGGA
GATTGGTTTGCTTGAAGAGTCAATATCTCGAAGGTTAAGTGTTTTAGAGGAGAAAGAGAT
TGAAACTGATTTGAGATTGGTCATTGAAGCAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGGTTATT
   
Sequence Name 03-K110036-0022-179-E12C-8409
GenBank Accession LN487531 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K110036-0022-179-E12C-8409
AAGCCATTTACAATTGAATATATCCTCGCTTCAATGACCAATCTCAAATCAGTTTCAATC
TCTTTCTCCTCTAAAACACTTAACCTTCGAGATATTGACTCTTCAAGCAAACCAATCTCC
TTCTCCTTCATTTCAACCACTCTCAGTCGTTCTTCCTTCTTCTCCAGCGAGGCGACCACG
TCATCAAACTGAAGCGAAAACTCAAAAATTGATGAAGATTTTGCCTGAATCTTGTCTAGG
ATTTTGAGATACCCTTCACTGTTTTGCTCAGTAACGTCTGATTCTACTGAATCCCCTTGC
ATTGCCATATTCTCAGAGACATAAACCCTACTTTTTAGTTAACAAAAGATGGGTCATAAA
GGAAGAAACTTTTCATGAAAAAATACAAAAATGAGAATGTACAGAAAGAATAAATAATAG
AAACAAAAAGTAGGAAGAGAAAATAAACCTCTCTTGAGTTTGGTCTTCGGTTGACACCAA
CGGTGGTTTCTGAGCCGGCGTGTCTTCTCCGGGAACTAGTAAA

Last Updated on 06.12.2018 16:11