Sequences from confirmation amplicon


Confirmation sequences for line 179F06 and hit At4g14700 are shown.
Line ID 179F06
Locus At4g14700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8422249
Primers used for PCR IF44 - 8474, show primer details
   
Sequence Name 50-K101385-0022-179-F06C-8409
GenBank Accession LN507107 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K101385-0022-179-F06C-8409
GCCATTTACATTGAATAATCCTGGACATGAGTTCCAAGGCAAAACCCTTTCAAATCCCCA
ACCAAAACTCCAACTAAAATGTATCGCATATCGTATCTATCTCCATCTTCAACTTCACTC
ACTCCTCCTCAAACCCCAGAAACTCTCACTCCTCTTCGTAGATCTTCTCGTCACGTTTCC
CGTAAAATCAATCTGGGAAATGACCCAATCGATCTTCCAGGAAAAGAATCAGTCGAAGAA
ATCAATCTAATTCGAAAACCTAGAAAACGAACCAATGATATTGTTGTTGCAGAGAAGTCG
AAGAAGAAGAAGATAGATCCGGAAGTTTCCTTCTCTCCTGTTTCACCAATTCGATCTGAG
ACGAAAAAGACGAAGAAGAAGA
   
Sequence Name 50-K101391-0022-179-F06C-IF44
GenBank Accession LN507108 [GenBank]
Sequencing primer gene specific primer, IF44: 5'-TTCTTCTTCTTCGTCTTCTTCGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K101391-0022-179-F06C-IF44
GAACTTCCGGATCTATCTTCTTCTTCTTCGACTTCTCTGCACAACAATATCATTGGTTCG
TTTTCTAGGTTTTCGAATTAGATTGATTTCTTCGACTGATTCTTTTCCTGGAAGATCGAT
TGGGTCATTTCCCAGATTGATTTTACGGGAAACGTGACGAGAAGATCTACGAAGAGGAGT
GAGAGTTTCTGGGGTTTGAGGAGGAGTGAGTGAAGTTGAAGATGGAGATAGATACGATTT
GCGATACATTTTAGTTGGAGTTTTGGTTGGGGATTTGAAGGTTTTTGCCTTGGAACTCAT
GTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
NAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11