Sequences from confirmation amplicon


Confirmation sequences for line 179H08 and hit At2g30370 are shown.
Line ID 179H08
Locus At2g30370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12941012
Primers used for PCR BF39 - 8474, show primer details
   
Sequence Name 19-K029004-022-179-H08C-8409
GenBank Accession LN494628 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K029004-022-179-H08C-8409
TTTACAATTGAATATCCGGACATGAACTCAATTTCAGCTGCTATCAACATAAGATAGTGA
GAGGACTGCGTTACTAACGTGCGATTTCATGCTGACATACGCATATTATTGTTAAAATGC
GCAGTTTAGGTAAAAAACATTTTTAAGACAGGGTTGGGGATGTGAGACAAGACGCGGGAA
AACCGCTCCGGAAAAAGCGCTACGCACATTACCAAAAAGGGCAGAGAGATTGAGAAGTTC
CAAGAGCCTGTCATACGTTAACGTGCACCGTGGGGGCTAATATTACCACTCGATCGAGAT
CGTAAGCAAGGCAGGGTCCCACGAGTGGTGAACGAACCAAAAGTTGAGTACTGTCCTTGT
CCTCGTGTGCGCTGAAACCAATCCGACGGCTGAGAATTTCTTTAGGAGTACATGGCATCT
CTGCATGCCCAGTCAGTCTCGACATGCCGTCACAGTCGCTGACTGTTCAAGCGCAGTTTA
GCCACAACCCTTTTGTT
   
Sequence Name 20-K029004-022-179-H08C-BF39
GenBank Accession LN495080 [GenBank]
Sequencing primer gene specific primer, BF39: 5'-GGACTACACAAACAAAAGGGTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K029004-022-179-H08C-BF39
GACGGCATGTCGAGACTGACTGGGCATGCAGAGATGCCATGTACTCCTAAAGAAATTCTC
AGCCGTCGGATTGGTTTCAGCGCACACGAGGACAAGGACAGTACTCAACTTTTGGTTCGT
TCACCACTCGTGGGACCCTGCCTTGCTTACGATCTCGATCGAGTGGTAATATTAGCCCCC
ACGGTGCACGTTAACGTATGACAGGCTCTTGGAACTTCTCAATCTCTCTGCCCTTTTTGG
TAATGTGCGTAGCGCTTTTTCCGGAGCGGTTTTCCCGCGTCTTGTCTCACATCCCCAACC
CTGTCTTAAAAATGTTTTTTACCTAAACTGCGCATTTTAACAATAATATGCGTATGTCAG
CATGAAATCGCACGTTAGTAACGCAGTCCTCTCACTATCTTATGTTGATAGCAGCTGAAA
TTGAGTTCATGTCCGGATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA
CGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCG
GAAATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11